摘要
为连续观察药物对细胞代谢的影响，用含紫杉醇的灌注液对人口腔上皮癌ＫＢ细胞和人结肠癌ＨＣＴ８细胞在灌注条件下进行了细胞３１Ｐ谱测定，从两种细胞在未给药、给药以及停药后的灌注条件下获得的代谢图谱中发现，紫杉醇使ＫＢ细胞和ＨＣＴ８细胞的ＡＴＰ代谢水平均有升高，但ＫＢ细胞的ＡＴＰ升高更为明显；同样剂量的长春新碱使ＫＢ细胞代谢中的ＡＴＰ峰的改变不明显，推测可能与它们的作用机制不同有关。研究结果表明，灌注细胞３１Ｐ谱测试法对观察药物对细胞代谢的作用是独特的。
31P NMR was performed for the studies on the metabolic changes of endogenous phosphoruscontaining molecules in KB and HCT8 cells exposed to taxol at a concentration of 10-6 mol·L-1. Using the perfusion method, the cells can be detected for a longer time by NMR, so as to give continuous spectra of the two celllines during the perfusion with and without the drug. The spectra showed that the levels of ATP peaks for both cells enhanced during the perfusion with the drug, but the change of the level is more prominent in KB cells than in HCT8 cells. This shows that KB cells are more sensitive to taxol than HCT8 cells. This is in coincident with the result in the cytotoxicity studies. However, the experiment using vincristine at the same concentrations demostrated that the level of the ATP peak was not changed significantly.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第2期117-120,共4页
Acta Pharmaceutica Sinica
