摘要
目的了解结核分支杆菌耐异烟肼（ＩＮH）分离株ＫａtG基因完全缺失或突变的情况，探讨其在ＩＮＨ耐药性中的可能作用。方法应用ＰＣＲ和ＰＣＲ－ＳＳＣＰ方法对５８株结核分支杆菌临床分离株的ＫａｔＧ基因进行分析。结果以Ｈ３７，ＲＶ标准株为对照，１２株敏感株的ＫａｔＧ基因扩增产物，１１株SSCP泳动正常，1株（８．３％）异常；２４株耐ＩＮＨ株中，３株（ｌ．５％）PCR扩增阴性，ＺＩ株ＫａtG扩增阳性，其中１６株（７６．２％）SSCP泳动异常；２２株耐其它抗结核药物株中，也有７株（３１．８％）SSCP异常。结论某些结核分支杆菌ＩＮＨ耐药性产生可能是由于其ＫａｔＧ基因完全缺失或ＫａｔＧ基因突变所致，但某些菌株也可能与ＫａｔＧ基因无关，是其它耐药基因所致或存在多种机制，尚需进一步探讨。
Objective To observe the mutations of katG in M. tuberculosis inoniazid - resident isolates, and to research their action in isoniazid resistance. Method Analyzing the katG gene in M. tuberculosis clinical isolates with PCR and PCR-SSCP. Results Of 12 drug-susceptible isolates, 11 katG ac products had no differences in the SSCP profiles with control. 7 hatG products of 22 non - isoniazid-resistant isolates had apparent SSCP difference. Of 25 isoniazid -resis- tant isolates,3 katG genes were not amplified, 16 katG PCR products showed apparent differences in the SSCP profiles. Conclusions Isoniazid resistance in some M. tuberculosis strains is associated with the complete deletion of katG gene or the mutations of katG gene. But some strains maybe have other gene alterations or other mechanism of isoniazid -resis- tance.
出处
《中国防痨杂志》
CAS
1998年第1期35-36,共2页
Chinese Journal of Antituberculosis
关键词
聚合酶链反应
药物耐受性
结核分枝杆菌
drug resistance polymerase chain reaction single - strand conformation polymorphism M. tuberculosis
