摘要
用细菌／杆状病毒（Ｂａｃ－ｔｏ－Ｂａｃ）系统在昆虫细胞中高效表达了绿色荧光蛋白（ＧＦＰ）与ＨＣＶ抗原的双功能融合蛋白，经ＥＬＩＳＡ测定和荧光显微镜观查证实，表达产物既能发射易于检测的绿色荧光，又具有ＨＣＶ的抗原活性，实现了用绿色荧光蛋白等分子标记抗原，为免疫诊断新方法的建立打下了理论基础．
Hepatitis C Virus(HCV) is most serious human viral disease now afflicting the worlds population.So,to develope a rapid and cheap method for early diagnose is always the main interest.The HCV core gene is labelled with gfp gene (green fluorescence gene) and the chimeric gene of gfp and HCV core was transposed to shuttle vector bacmid by Bac to Bac system and a bi function fusion protein was expressed in insect cells.The fusion protein was determined by ELISA kit,meanwhile the intensity of luminescence was observed under the fluorescent microscope.The results have demonstrated that the expressed GFP HCV core fusion protein not only can generate striking green fluorescence but also have antigenic activity.The approach which the antigen was tagged by equal molecular green fluorescent protein allow us to use the bi function fusion protein to establish a new method for immunological diagnosis.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1998年第2期134-139,共6页
Chinese Journal of Biochemistry and Molecular Biology
基金
世界银行贷款项目
国家教委博士点基金
关键词
HCV
核心蛋白
基因
绿色荧光蛋白
抗原融合蛋白
HCV core gene
gfp gene
Bi function fusion protein
Bac to Bac system
Clone and expression
