摘要
本文报导一种富集和分离根际联合固氮芽孢杆菌的方法，该方法以果胶为唯一碳源的培养基进行富集，中性红为指示剂的培养基进行分离，根据固氮芽孢杆菌能产气以及在分离培养基平板上菌落为红色两个重要的鉴别性特征即可检出，准确性高，简便快速；应用此法从１５个地点６种作物共８８个根系样品中分离出５１株固氮芽孢杆菌，经鉴定均为多粘芽孢杆菌（Ｂａｃｉｌｕｓｐｏｌｙｍｙｘａ）；还初步讨论了多粘芽孢杆菌在几种作物根际的分布情况。
In this article, a new method for enriching and isolating the nitrogenfixing Bacillus from plant rhizosphere was reported. The medium containing pectin as the sole carbon source was used to enrich the nitrogenfixing Bacillus. The medium using neutral red as an indicator was used to isolate the nitrogenfixing Bacillus. The nitrogenfixing Bacillus were selected according to their two important characters:they could produce gas and their colonies were red on the isolation medium plate. Using this method, 51 nitrogenfixing Bacillus strains were isolated form 88 root samples of 6 plants on 15 spots. They were all identified as B. polymyxa. The distribution of B.polymyxa in some plants rhizospheres was discussed as well.
出处
《氨基酸和生物资源》
CAS
1998年第1期12-15,共4页
Amino Acids & Biotic Resources
关键词
固氮芽孢杆菌
富集
分离
鉴定
生态
nitrogenfixing Bacillus, enrich, isolate, identify, distribution
