摘要
用多发性骨髓瘤病人血清中提纯的ＩｇＭ抗原，用脾内免疫ＢＡＬＢ／ｃ小鼠后与Ｓｐ２／Ｏ骨髓瘤细胞融合，获得了７株分泌人ＩｇＭ单克隆抗体的杂交瘤细胞株，分别命名为２５Ｇ１、２５Ｇ３、２５Ｇ４、２５Ｇ５、２５Ｄ２、２５Ｄ３和２５Ｄ５。注射同系小鼠后可诱生含较高效价抗人ＩｇＭ腹水（ＰＨＡ＝１２１２）。该杂交瘤细胞经组织培养传代半年，冻存１４个月后复苏，其分泌ＩｇＭ性能稳定。用ＰＨＡ、ＥＬＩＳＡ做人ＩｇＭ、ＩｇＧ、ＩｇＡ阻断试验，仅ＩｇＭ可阻断。
IgM antigen was isolated from human serium with multiple myeloma.Monoclonal antibodies (McAbs) against human IgM were produced by fusing sp2/0 myeloma cells with spleen cells from BALB/C mice immunized with IgM by means of intraspleen immunization.Seven hybridomas,which were named 25C1、25C3、25C4、25C5、25D2、25D3 and 25D5 respectively,constantly secret specific antibodies against human IgM.The specificity of the McAbs against IgM was confirmed by immunoelectrophoresis double diffusion test,PHA and ELISA.No reactivity was detected with IgM and IgG by PHA and ELISA.Mouse ascitic fluids producted by these hybridomas had antibody titers against human IgM of 1∶212 by PHA.The hybridoma cell strains lasted for half a year by way of passage and were freezed in presence of liquid nitrogen for 14 months,then were thawed.It was found that hybridoma cell strains can stably keep the function of IgM secretion.
出处
《微生物学免疫学进展》
1998年第1期66-68,共3页
Progress In Microbiology and Immunology
关键词
IGM
单克隆抗体
细胞融合
杂交瘤
Anti-Human IgM McAb Cell fusion Hybridoma cell strains
