摘要
采用聚丙烯酰胺凝胶电泳技术 ,对同一样地的不同时间和空间土壤中的野生大豆 (Glycinesoja)种子库内的 4种同工酶进行了分析 ,并利用数量分类学方法和统计学上的X2 测验 ,初步探讨了野生大豆种子库在此 4种同工酶水平上的遗传多样性的时空动态 .研究表明野生大豆种子库在同工酶水平上呈现出遗传多样性 ,其主要来自于由时间引起的环境异质性 .在时间变化上 ,同一层次的多样性呈现出极显著的差异 .在空间变化上 ,不同层次的多样性发生着不同的变化 .层与层之间同工酶的差异随时间的推移而加大 ,而且同一层次野生大豆种子库的平均杂合度也呈现出同样的变化规律 .这说明在对野生大豆种子库同工酶水平上的遗传多样性的影响程度上 ,时间比空间起着更大的作用 .
Using polyacrylamide gel electrophoresis (PAGE) technigue, this paper analyzed four isozymes in the seed bank of Glycine soja, and the temporal and spatial dynamics of genetic diversity at isozyme level were studied. Isozymatic analysis, cluster analysis and X 2 test revealed that genetic diversity based on the four isozymes existed in the seed bank of G.Soja, and that the diversity mainly originated from environmental difference caused by time. The diversity in the same layer showed a significant difference with time. The seeds scattered in different deep showed different change in their genetic diversity. The variations of isozymes within layers became more and more distinct with time, and the average heterozygosity of seed bank in the same layer appears the same pattern. The results suggest that time takes more significant effect on genetic diversity at isozyme level in the seed bank than space does.
出处
《应用生态学报》
CAS
CSCD
1998年第2期145-149,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金资助项目 !( 3 92 3 0 2 50 )
关键词
野生大豆
种子库
同工酶
遗传多样性
Glycine soja, Seed bank, Isozyme, Genetic diversity.
