摘要
在缺氧－再给氧条件下，观察了体外分离培养的大鼠脑微血管内皮细胞表面粘附分子ＩＣＡＭ－１的表达及中性粒细胞与内皮细胞粘附作用的改变。结果表明，单纯缺氧１０ｈ不引起内皮细胞ＩＣＡＭ－１的上调，再给氧６ｈＩ－ＣＡＭ－１的表达升高（Ｐ＜０．０１），再给氧１２ｈ表达量增加了１００％（Ｐ＜０．０１），此时中性粒细胞与内皮细胞的粘附作用也明显增强（Ｐ＜０．０１）。缺氧前用盐酸川芎嗪（２ｍｇ／ｍｌ）预处理内皮细胞可阻断ＩＣＡＭ－１的表达（Ｐ＜０．０１），同时也可降低ＰＭＮ与内皮细胞的粘附（Ｐ＜０．０５）。结果提示，脑微血管内皮细胞在缺氧－再给氧刺激下可自身调节Ｉ－ＣＡＭ－１的表达，为中性粒细胞与内皮细胞的粘附提供特异的结合位点。
? We undertook this study to determine where was increased endothelial expression of ICAM1 and the change of neutrophilendothelium interaction during hypoxiareoxygenation.Results showed that 10h periods of hypoxia did not lead to endothelial ICAM1 expression as measured by laser confocal microscope.ICAM1 dramatically increased at 6h of reoxygenation and doubled at 12h of reoxygenation,while the binding of neutrophils to endothelial cells also increased significantly.The 12h increase levels of both ICAM1 expression and adhesion of neutrophils were reduced with the addition of 2mg/ml Ligustrazine at the time of hypoxia.These data indicate that endothelial cells can self uprgeulate the ICAM1 expression under hypoxiareoxygenation,which provides specific position to facilitate neutrophil adhesion.
出处
《中国应用生理学杂志》
CAS
CSCD
1998年第1期14-16,共3页
Chinese Journal of Applied Physiology
