摘要
高效毛细管电泳以其快速、高效、简便、高分辨率、样品和溶剂消耗少及易于仪器化等优点正成为生物活性分子分离和分析工作中的一个重要工具。介绍了作为四大基本生命物质之一的糖类化合物的高效毛细管电泳研究进展,包括CZE、CGE、MECC和CITP等四种分离模式以及直接紫外/荧光法、间接紫外/荧光法、示差法、电化学法和质谱法等五种检测方式。并简要介绍HPCE在单糖、寡糖和多糖分离与分析中的应用,展望了HPCE用于单个细胞中糖类组分的研究前景。
High Performance Capillary Electrophoresis(HPCE) is rapidly gaining acceptance as a modem powerful analytic tool for the study of bioactive molecules, because of its capability of rapid, tremendous efficiency, simply, reproducible, automated and extremely high- resolution separation of complex mixtures of diverse size and nature in very small sample volumes. In this review we in troduced the wide application of HPCE on carbohydrates, one of the most basic life substances, and discussed the approaches recently developed for overcoming the separating and detecting difficulties in electrophoresis, including four separating modes- CZE, CGE, MECC, and CITP, and five detection methods, such as direct UV/Fluorescence detection, indirect UV/Fluorescence detection, refractive index detection, amperometric detection and mass spectroscopy method. In the end, we also predict the future application of this promising technique in ultramicro - analysis of carbohydrates in a single cell.
出处
《有机化学》
SCIE
CAS
CSCD
北大核心
1998年第1期88-96,共9页
Chinese Journal of Organic Chemistry
关键词
糖类
毛细管电泳
单糖
寡糖
多糖
HPCE
carbohydrate, capillary electrophoresis, monosaccharide, oligosaccharide, polysaccharide
