摘要
研究了小核盘菌保藏过程中活力和致病力影响因素,结果表明:接种后培养6 d的小核盘菌活力(以该菌在PDA平板上培养形成的菌落直径表示)和致病力(以该菌在蒲公英叶片上形成的菌斑直径表示)最大,分别为66.2 mm和52.9 mm。保藏期间小核盘菌的活力和致病力均随时间呈下降趋势。保藏90 d后,培养8 d的小核盘菌的活力和致病力最大,分别为31.7 mm和17.9 mm;培养4 d的小核盘菌的活力和致病力最小,分别为6.3 mm和2.6 mm。保藏期间低水活度有利于小核盘菌活力和致病力的保持,活力和致病力均随水活度的降低而增加。当水活度低于0.432时,活力和致病力不再增加,趋于稳定。低温有利于小核盘菌活力和致病力的保持,25℃下保藏90 d的小核盘菌失去了全部活力和致病力,而-16℃下小核盘菌的活力和致病力仅分别下降25.8%和13.7%。
Right after inoculation by Sclerotinia minor, the herbicide stored for 6 days had the highest viability and pathogenicity, with a diameter of 66. 2 mm of the colony on PDA plate and 52. 9 mm of the lesion on dandelion leaves, respectively. Both the viability and pathogenicity declined during storage. The herbicide stored for 8 days had the highest viability and pathogenicity 90 days after storage, with a diameter of 31.7 mm and 17.9 mm, respectively, while the lowest viability and pathogenicity were observed if the herbicide stored for 4 days, with a diameter of 6. 3 mm and 2.6 mm, respectively. Low water activity favored long-term viability and pathogenicity, which increased as water activity fell. But when water activity was lower than 0. 432, the viability and pathogenicity remained stable. At lower temperature, the herbicide kept higher viability and pathogenicity. The herbicide totally lost its viability and pathogenicity at 25 ℃ 90 days after storage.
出处
《植物保护》
CAS
CSCD
北大核心
2009年第2期85-88,共4页
Plant Protection
基金
黑龙江省科技计划项目(KT05A400-1)
哈尔滨市科技攻关计划项目(2007AA6CN105)
东北农业大学创新团队发展计划项目(CXT003-2-1)
关键词
小核盘菌
活力
致病力
保藏
Sclerotinia minor
viability
pathogenicity
storage
