摘要
阐述了含有五氯苯酚（ＰＣＰ）及２，４－二氯苯酚（ＤＣＰ）的白腐真菌降解液的预处理以及利用高效液相色谱二极管阵列（ＰＤＡ）检测器快速测定ＤＣＰ和ＰＣＰ的方法。实验表明，在如下色谱条件：ＮｏｖａｐａｋＯｄｓ柱，流动相８０％甲醇，２０％００３ｍｏｌ／ＬｐＨ＝２７磷酸缓冲溶液（Ｖ／Ｖ），流速０８ｍＬ／ｍｉｎ，检测波长３００ｎｍ时，ＰＣＰ和ＤＣＰ在２０～１００μｇ／ｍＬ浓度范围内，其吸收和浓度具有良好的线性关系。
The liquid with pentachlorophenol(PCP) and 2,4-dichlorophenol(DCP) that is degraded by white rot fungus has been prehanded,PCP and DCP can be analyzed quickly as the followed conditions: Novapak Ods HPLC column, the mixture of methol and 003mol/L phosphoric acid buffer (pH=27)(80:20, V/V) as the mobile phase, the flow rate is 08mL/min, and the detecting wavelength is 300nm.It's found that when the concentration of DCP and PCP range between 20～110μg/mL,the absorbance are proportional to their concentration quite properly.
出处
《江苏石油化工学院学报》
1998年第1期42-44,共3页
Journal of Jiangsu Institute of Petrochemical Technology
基金
中国石化总公司资助研究项目
关键词
液相色谱仪
五氯苯酚
白腐真菌
废水处理
降解
High performance liquid chromatography
Photodiode array detector
Pentachlorophenol
2,4-dichlorophenol
White rot fungus
