摘要
目的:建立高效液相色谱法测定利福霉素钠含量的。方法:采用高效液相色谱法,色谱柱为KmmαsilC_8 , (甲醇:乙腈:0075 mol/L磷酸二氢钾溶液:1.0mol/L枸橼酸溶液)=50:22.5:22.5:5为流动相,流速为1. 0 ml/min,检测波长为254nm。结果:利福霉素钠含量在(41.02～164.08) μg/mL范围内线性关系良好(r=1.0001),平均回收率为99.79% , RSD为1.56%,规定含量限度为90.%-105.0%结论:本法准确、灵敏、可靠,能有效地控制该制剂的质量。
Objective： To establish a HPLC method for the determination of rifamycin sodium. Methods： Using high performance liquid chromatography,column KromasilC8,（methanol： acetonitrile： 0075 mol / L potassium dihydrogen phosphate solution： 1.0 mol / L citrate solution）=50∶22.5∶22.5∶5 The flow rate was 1.0 ml / min,detection wavelength at 254nm. Results： The sodium content of rifamycin（41.02 - 164.08）μg / mL range a good linear relationship（r=1.0001）,the average recovery was 99.79%,RSD 1.56%,the content limit of the provisions of 90.0%-105.0%. Conclusion： This method is accurate,sensitive,reliable,able to effectively control the quality of the preparation.
出处
《中国医药导刊》
2009年第1期161-162,共2页
Chinese Journal of Medicinal Guide
关键词
高效液相色谱法
利福霉素钠
含量测定
High performance liquid chromatography
Rifamycin sodium
Content determination
