摘要
ＴＡＫ（Ｔａｃｔｉｖａｔｅｄｋｉｌｅｒ，ＴＡＫ）细胞是由抗ＣＤ３单克隆抗体和ｒＩＬ２共同激活诱生的具有高度溶细胞活性的肿瘤杀伤效应细胞。为探讨ＴＡＫ细胞的免疫生物学特征，研究了ＴＡＫ细胞的形态学和细胞表型。结果表明，ＴＡＫ细胞体积较大，核／浆比例小，胞浆内含有大量颗粒，具有活化淋巴母细胞的形态学特征；９０％以上的ＴＡＫ细胞表达Ｔ淋巴细胞表型（ＣＤ３＋，ＣＤ８＋），２０％～５０％细胞为ＮＫ细胞表型（ＣＤ１６＋，ＣＤ５６＋），并表达ＩＬ２受体、ＣＤ３８和ＭＨＣⅡ类抗原等激活淋巴细胞的表面标志。攻击Ｋ５６２细胞的ＴＡＫ细胞主要为富含颗粒、ＣＤ８＋和ＣＤ５６＋细胞。证明ＴＡＫ细胞是以激活Ｔ淋巴细胞和ＮＫ细胞为主的具有细胞毒活性的异质性细胞群体。
おactivated killer (TAK) cells were tumorkilling effectors with high cytolytic activity induced by antiCD3 monoclonal antibody and interleukin2 coactivation. In order to take their biological features the cytomorphology and phenotypes of TAK cells were studied. The results showed that TAK cells were morphologically similar to these of lymphoblasts and more than 90% of TAK cells were phenotypes of T lymphocytes (CD3+,CD8+).Some of TAK cells expressed IL2 receptor, CD38 and MHC Ⅱ antigens characteristic makers for the activated T lymphocytes. TAK cells which attacked the leukemia K562 cells were morphologically large volume with granules, and mainly contained CD8+ and CD56+ cells. The study suggested that TAK cells are heterogeneous cell population with cytotoxicity and consisting of mainly activated T lymphocytes and NK cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第4期260-262,共3页
Chinese Journal of Immunology
基金
甘肃省中青年科技基金
关键词
T-AK细胞
形态学
细胞表型
TAK cells Cytomorphology Phenotype Heterogeneity
