摘要
综述了目前用于赤潮藻的分子生物学检测技术,这些技术总体分为3类,即基于核酸提取的检测技术、全细胞杂交技术和酶联免疫吸附分析技术(ELISA)。分子生物学检测技术能用于自然水样的种群分析、有害赤潮藻种的日常监控、赤潮藻孢囊的鉴别和对压舱水外来生物的检测等。今后研究的重点是如何将各种已建立的检测技术运用于实践,特别是如何提高检测的速度、灵敏度,并发明新的检测设备,实现检测自动化。
This paper reviews the molecular methods for detection of red tide-causing alag species, which outperform other kinds of approaches because of their advantages in simplicity, speediness, precision and specificity. The molecular techniques can be classified into three categories ： the detection based on extraction of DNA, the whole cell hybridization, and the enzyme-linked immunosorbent assay （ELISA）. Molecular methods can be extensively applied to many fields, including natural population analysis, daily monitoring of harmful algae, identifying spores and detecting ballast water. The future work should emphasize on how putting the established methods into practice, improving the detection rapidity and sensitivity, and inventing new detection instruments for detection automation.
出处
《高技术通讯》
EI
CAS
CSCD
北大核心
2009年第3期314-322,共9页
Chinese High Technology Letters
基金
863计划(2007AA092110)
国家自然科学基金(U0633006)
哈尔滨工业大学(威海)研究基金(HIT(WH)07)
哈尔滨工业大学科研创新基金(IMOO29080006)
城市水资源与环境国家重点实验室开放课题(ES200802)资助项目
关键词
赤潮
赤潮藻
鉴定
检测
分子生物学
red tide, red tide algae, identification, detection, molecular biology
