摘要
目的探讨间质性肺疾病（ＩＬＤ）时中性粒细胞趋化因子（ＮＣＦ）和肿瘤坏死因子（ＴＮＦα）与ＩＬＤ活动性的关系。方法用膜滤过和放射免疫法检测１１例结节病、７例特发性肺间质纤维化（ＩＰＦ）患者和８名健康者血清及支气管肺泡灌洗液（ＢＡＬＦ）中ＮＣＦ活性及ＴＮＦα水平。结果７例ＩＰＦ患者ＢＡＬＦ中ＮＣＦ、ＴＮＦα分别为２０３±４４ｃｅｌｓ／１０ＨＰ、１１７±２９ｎｇ／Ｌ，明显高于８名对照组（８３±４５ｃｅｌｓ／１０ＨＰ、６５±１４ｎｇ／Ｌ、Ｐ＜０．０１）；１１例结节病患者ＢＡＬＦ中ＮＣＦ、ＴＮＦα分别为１８６±５０ｃｅｌｓ／１０ＨＰ、１２±３ｎｇ／Ｌ，明显高于８名对照组（Ｐ＜０．０１）。ＩＰＦ组ＢＡＬＦ中ＮＣＦ、ＴＮＦα均与中性粒细胞百分比呈正相关（ＮＣＦ：ｒ＝０．８９，Ｐ＜０．０１，ＴＮＦα：ｒ＝０．８６，Ｐ＜０．０５），结节病组ＢＡＬＦ中ＮＣＦ、ＴＮＦα均与淋巴细胞百分比呈正相关（ＮＣＦ：ｒ＝０．７８，Ｐ＜０．０１；ＴＮＦα：ｒ＝０．７３，Ｐ＜０．０１）。结论ＩＰＦ和结节病患者ＢＡＬＦ中ＮＣＦ。
Objective To investigate the relationship between the level of the neutrophil chemotactic factor(NCF)、tumor necrosis factor α(TNF α) in patients with interstitial lung disease(ILD)and the activity of ILD. Method The NCF activities in the BALF and in the serum from 11 patients with sarcoidosis, 7 with idiopathic pulmonary fibrosis (IPF)and 8 normal subjects were determined using the membrane filter and radio immunoassay. The level of TNF α was also detected. Result In the 7 IPF patients, the level of NCF and TNF α (203±44 cells/10HP, 11.7± 2.9 ng/L) in the BALF was higher than that in 8 control patients (83±45 cells/10HP,6.5±1.4 ng/L, P <0.01). The level of NCF and TNF α in the BALF from 11 patients with sarcoidosis (186±50 cells/10HP, 12±3 ng/L) was highet than those in 8 control patients ( P <0.01). The level of NCF and TNF α in the BALF from patients with IPF was positive correlated with the percentage of neutrophil (NCF: r =0.89, P <0.01; TNF α: r =0.86, P <0.05). The level of NCF and TNF α in the BALF of patients with sarcoidosis was positive correlated with the percentage of lymphocyte (NCF: r =0.78, P <0.01; TNF α: r =0.73, P <0.01. Conclusion The level of NCF and TNF α in the BALF from patients with IPF and sarcoidosis can act as the marker of the activity of alveolitis of IPF and sarcoidosis.
出处
《中华结核和呼吸杂志》
CSCD
北大核心
1998年第5期297-299,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
卫生部医学重点科技项目基金
关键词
肺疾病
间质性肺疾病
NCF
肿瘤坏死因子
Interstitial lung disease
Broncho alveolar lavage fluid
Neutrophil chemotactic factor Tumor necrosis factor α
