摘要
目的:了解安徽马鞍山市肠道病毒71型(EV71)基因型的分布状况。方法:利用逆转录半巢式PCR(RT-nPCR)的方法检测马鞍山地区40份临床诊断为手足口病患儿的血清和咽拭子标本。结果:40份标本中检测到2份阳性EV71 RNA,检出率为5%;经过测序证实2份标本均为EV71 C型。结论:马鞍山地区手足口病主要由C型EV71病毒引起;利用生物信息学软件对这2份标本的核苷酸同源性进行比较,发现C型EV71存在一定的变异。
Objective：To investigate the EV71 genotypes in Maanshan area.Methods：EV71 RNA was detected in 40 samples from the hospitals with reverse translation polymerase chain reaction（RT-nPCR）.Results：There were only 2 positive products,positive rate was 5%.After the 2 positive products were sequenced,the results showed that they were of C genotype EV71.Conclusion：It seems that the HFMD are mainly infected by the C genotype EV71 in Maanshan area.The sequence comparisions of the positive products show homogeneous not very high.This indicats that the current C genotype EV71 varies significantly.
出处
《中国卫生检验杂志》
CAS
2009年第3期594-595,657,共3页
Chinese Journal of Health Laboratory Technology
