摘要
目的：建立固相萃取-气相色谱法测定蔬菜、水果中15种有机磷农药残留量的分析方法。方法：蔬菜、水果捣碎经乙腈提取,浓缩,CARB/NH2柱净化,丙酮溶解定容,用CP-SIL 24CB毛细管柱和TSD检测器测定。结果：相关系数为0.9966～0.9999,15种有机磷农药回收率加标0.080 mg/kg时,为80.0%～111.2%之间,加标0.20 mg/kg时,为85.0%～108.5%之间,加标4.00 mg/kg时,为97.2%～103.5%之间,变异系数RSD,加标4.00 mg/kg时,为2.21%～6.39%之间。结论：实验结果表明,在选定的色谱条件下,采用固相萃取-气相色谱法对蔬菜、水果中15种有机磷农药残留量进行测定。本法线性范围宽,线性良好,重现性好,回收率很高,能满足有机磷农药残留量测定,完全能够达到国家标准方法的要求。
Objective：To establish a method for the simultaneous determination of 15 organophosphorous pesticide residues in vegetables and fruits by gas chromatography（GC）.Methods：The residues was determined by TSD neutral detector with CP-SIL 24CB capillary column after acetonitrile extraction,salting-out,concentration,CARB/NH2 column purification,and dissolved to constant volume with acetone.Results：The recoveries varied between 80.0%～111.2% respectively when 0.080 mg/kg standard solution were added in,85.0%～108.5% respectively when 0.20 mg/kg standard solution were added in and 97.2%～103.5% respectively when 4.00 mg/kg standard solution were added in.RSD was between 2.21%～6.39% when 4.00 mg/kg standard solution were added in.Conclusion：This method has reliable producitivity and can give a wide linear range with good recovery and RSD.So it is suitable for the analysis of organophosphorous pesticide residues in vegetables and fruits.
出处
《中国卫生检验杂志》
CAS
2009年第3期571-573,共3页
Chinese Journal of Health Laboratory Technology
关键词
蔬菜
水果
农药残留量
固相萃取
气相色谱法
Vegetable
Fruits
Organophosphorous pesticide residues
Solid phase extraction（ SPE）
Gas chromatography（GC）
