摘要
１９９３年在大兴安岭林区，从落叶松毛虫（ＤｅｎｄｒｏｌｉｍｕｓｓｕｐｅｒａｎｓＢｕｔｌｅｒ）自然死亡虫体内分离到一株病原细菌，经过分离、纯化、培养，这是一株能产生芽孢和伴胞晶体的苏云金杆菌（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓ），定名为Ｌ－９３菌株。通过生理生化反应试验和血清学鉴定，确定该菌的血清型为Ｈ３ａ３ｂ，但生化反应与库斯塔克变种略有不同，其特点是尿酶反应为阴性。对落叶松毛虫幼虫回接试验，５ｄ校正死亡率达９７２％。该菌株的发现为在寒冷地区开展生物防治森林害虫提供了一个优良菌株。
A bacteria plant was separated from the natrural dead D superans in the Daxing′anling forest region in 1993 By proving it is the Bacillus thuringiensis which can produce spore and crystal It is named as L-93 bacteria It′s blood serum type is H 3a3b , But it′s biochemistry reaction is different from the known H 3a3b B thuringensis var kurstaki It′s urease reaction is negative The dendrolimus correct death rate for five days is 97 2% It′s a good bacteria plant for the prevention and control of forestry diseases in cold region
出处
《东北林业大学学报》
CAS
CSCD
北大核心
1998年第1期70-74,共5页
Journal of Northeast Forestry University
基金
林业部重点课题
关键词
苏云金杆菌
松毛虫
生理生化反应
血清学
落叶松
Bacillus thuringiensis
Dendrolimus superans
Physiological and biochemistry reaction
Serological text
