摘要
以草鱼和鲤为材料，提取基因组ＤＮＡ，制备ＲＡＰＤ－ＰＣＲ模板，对３个随机引物盒共６０个１０－ｍｅｒ引物进行了ＰＣＲ扩增，共筛选出７个较理想的可用于鱼种内或鱼种间群体遗传分析的随机引物．用这７个引物所获得的草鱼和鲤的ＲＡＰＤ指纹图谱平均带纹总数分别为４．２８±１．８９和６．７１±４．１９，多态性带纹总数分别为１．７１±１．６０和５．１４±４．７４．ＲＡＰＤ指纹图谱特征反映出草鱼比鲤具有更高的遗传纯度．７个引物共扩增出草鱼特异性带纹１８条，鲤特异性带纹３２条．大量特异性带纹的检出表明，两个鱼种间存在较大的遗传差异。
Genomic DNA was extracted as RAPD PCR template from the liver tissue of grass carp and common carp, 60 10 mer random primers were used and 7 primers were proved to be ideal for population genetic analysis within and or between the fish species. The average number of total RAPD bands amplified with these 7 primers for grass carp and common carp were 4 28±1 89 and 6 71±4 19,respec tively, the average number of polymorphic bands were 1.71±1.60 and 5.14±4.74, respectively. The RAPD patterns suggested that the grass carp have higher genetic homogeneity than the common carp. 18 grass carp specific bands and 32 common carp specific bands were produced by all 7 primers, which implied significant genetic variation between the grasscarp and the common carp,and provided the candidate genetic markers for molecular assay of the gene transfer (especially whole genomic DNA transfer) between these two fish species.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
1998年第2期137-142,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科委资助
关键词
草鱼
鲤
随机引物
遗传标记
分子标记
ctenopharyngodon
idellus
cyprinus carpio
random primer
genetic marker
genetic analysis
