摘要
作者建立了山竹壳原料和山竹壳提取物中α-倒捻子素的高效液相色谱测定方法。采用WaterssymmetryC-18色谱柱（5μm，D4．6mm×150mm），流动相为甲醇-水（梯度洗脱），检测波长280nm，体积流量1．0mL／min，柱温为30℃。结果为α-倒念子素质量浓度在28．952～231．6μg／mL的范围内呈良好线性关系（r=0．9995），其回收率在98．77％～101．39％。用高效液相色谱法测定山竹壳原料和山竹壳提取物中α-倒捻子素的含量操作简便、结果准确、重复性好，可用于指导原料的采购和提取物质量指标的控制。
An assay method of a-mangostion content in mangosteen fruit skin and mangosteen fruit skin extract by HPLC was developed in this manuscript. Waters symmetry C-18 column（5μm,D4.6mm×150mm） was used as stationary phase and methanol/water was used as mobile phase with 280 nm for detection wavelength. The flow rate was 1.0 mL/min and the column temperature was 30 ℃. External standard method was apply to quantitative analysis. The linear range of α- mangostin was 28. 952 -231.6 μg/mL （ r= 0. 999 5） . The recovery was from 98. 77% to 101.39% . The method is simple, accurate, stable and reproducible , and can be applied to control of mangosteen fruit skin and mangosteen fruit skin extract.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2009年第2期256-258,共3页
Journal of Food Science and Biotechnology
