摘要
Open vertebroplasty for the management of spinal metastases and multiple myeloma, Surgical treatment of bone tumors of the shoulder girdle,Use of SELDI-TOF-MS method to identify patients with osteosarcoma,The value of MRI for the iagnosis and treatment of hemangiomas.
209370 Gene profiling of MAPK pathway in human osteosarcoma/Li Guodong(李国东,Dept Orthop,10th Peop Hosp Affil Tongji Univ,Shanghai 200433)…∥Chin J Oncol.-2009,31(5).-340～345Objective To explore the functional effects of MAPK pathway in the pathogenesis of human osteosarcoma.Methods Gene microarray(Human Genome U133A,Affymetrix)was used to screen the differential expression of genes involved in MAPK pathway between osteosarcoma cell lines and 3 osteoblastic cell lines.KEGG metabolic pathway analysis was performed among significantly increased or decreased genes using the MATLAB software.Immunohistochemical technique was used to detect the expressions of ERK1/2,JNK and p38 proteins among 48 osteosarcoma and benign 24 osteoblastic tumor samples.Results Using an entrance limit of ≥2.0,18 differentially expressed MAPK pathway-related genes were selected(10 up-regulated,8 down-regulated)to mapped to the MAPK pathway of KEGG which are all important node genes.The positive rates of ERK1/2,JNK and p38 proteins were 83.3%(40/48),72.9%(35/48)and 85.4%(41/48)in osteosarcomas,and 12.5%(3/24),8.3%(2/24)and 16.7%(4/24)in the control group,respectively.The positive rates and expression intensities were statistically different between the 2 groups(P<0.01).Conclusion MAPK pathway plays an important role in the pathogenesis of osteosarcoma.ERK,JNK and p38 form and intercoodinating network and regulate the cell proliferation,differentiation,apoptosis,invasion and migration in osteosarcoma.15 refs,2 figs,2 tabs.
