摘要
目的:体外分离培养骨髓间充质干细胞(MSC),并诱导其向神经元细胞分化。方法:分离大鼠骨髓间充质干细胞,并于体外扩增、培养,并通过诱导培养基进行体外诱导培养,比较对照组及实验组间细胞形态和神经丝蛋白(NFP)表达率的不同。结果:诱导后,实验组中80%的细胞表现出神经元样形态,且NFP阳性表达率明显高于对照组。结论:MSC在体外可以被诱导为神经元样细胞。
Objective：Mesenchymal stem cell（MSC）was isolated and grown in vitro,and induced to be differentiated into neuron-like cell.Methods：MSC was isolated,grown and induced in vitro.The comparison of induced cell morphology and expression of neurofilament protein（NFP） was carried out between control and experimental groups.Results：More than 80% of MSC exhibited neuron-like characteristics.NFP-positive cell in experimental group is higher than that in the control group（P〈0.05）.Conclusion：MSC can be induced to differentiate into neuron-like cell in vitro.
出处
《内蒙古医学院学报》
2009年第1期19-23,共5页
Acta Academiae Medicinae Neimongol
基金
内蒙古自治区教育厅资助项目(NJ02084)
关键词
骨髓间充质干细胞
诱导
分化
神经元样细胞
mesenchymal stem cell
induction
differentiation
neuron-like cell
