摘要
目的研究低氧条件下乳酸脱氢酶抑制剂2-脱氧-D-葡萄糖(2-DG)对胰腺癌细胞panc-1的影响。方法建立细胞体外低氧培养模型。应用MTT法检测不同浓度2-DG(0,0.5,1.0,2.0,3.0 mg/mL)对癌细胞增殖的影响;以流式细胞仪检测不同浓度干预下细胞周期的变化及其细胞凋亡率。结果低氧培养下,不同浓度2-DG对胰腺癌细胞panc-1生长均有明显抑制作用(P<0.0 5),并呈剂量依赖性;细胞凋亡率也明显增加(P<0.0 5),且细胞滞留于G2/M及S期。结论低氧条件下,2-DG能明显抑制人胰腺癌细胞的生长,并可阻滞细胞周期及诱导细胞凋亡。
Objective To investigate the effect of 2-Deoxy-D-glucose on the growth, cell cycle and apoptosis of pancreatic cancer cell panc-1 under hypoxic condition. Methods The panc-1 cell was cultured under hypoxic condition with in vitro model for 48 h. MTF, Hochest and PI staining, and fluorescence microscopy were used to detect the inhibition of the growth, apoptosis and cell cycle effect of 2-DG （0 mg/mL, 0.5 mg/mL, 1 mg/mL, 2 mg/mL and 3 mg/mL）. Results 2-DG had sgnificant concertration-dependent inhibition on the growth, apoptosis and cell cycle of pancreatic cancer cell （ P 〈 0. 05 ） . Flow cytometry （ FCM ） and Hochest showed that the proportion of the apoptosis increased gradually, and was concertration-dependent （ P 〈 0.05 ）. Conclusions 2- DG can inhibit the growth of human pane-1 cell by blocking the cell cycle and induce apoptosis under hypoxic condition.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第3期243-246,共4页
China Journal of General Surgery
