摘要
目的探讨急性胰腺炎肠道功能损伤时内毒素和D-乳酸的变化。方法Wistar大鼠随机分为假手术(SO)组和重症急性胰腺炎(SAP)组。术后分4个时段心脏抽血处死,取胰腺组织和回肠组织行组织学检查。偶氮显色法测定血浆内毒素;改良分光光度法测定血浆D-乳酸。结果SAP组血浆内毒素和D-乳酸随发病时间的延长而持续升高,SO组血浆内毒素和D-乳酸随时间的延长无明显变化。两组比较差异显著(P<0.05)。内毒素与D-乳酸呈正相关。(r=0.7323,P<0.05)。结论内毒素和D-乳酸在SAP肠道损伤时升高,可作为评价肠道屏障功能的指标。
Objective To explore the changes of plasma endotoxin and D-lactate during intestinal mucosal barrier injury of acute pancreatitis. Methods Wistar rats were randomly divided into sham-operation （ SO ） group, and severe acute pancreatitis （ SAP ） group. Plasma endotoxin and D-lactate were determined at 1 , 3,6 and 12 hour after operations. Results The plasma endotoxin and D-lactate in SAP group increased obviously with time, and the difference betueen the 2 groups was significante （ P 〈 0.05 ）. Endotoxin and D-lactate were positively correlated. Conclusions The plasms endotoxin and D-lactate are increased during gut injury of SAP, and can be used as indexes to evaluate the function of the intestinal mucosal barrier.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第3期224-227,共4页
China Journal of General Surgery
关键词
胰腺炎
急性坏死性
内毒素
D-乳酸
肠道屏障
Pancreatitis, Acute Necrotizing
Endotoxin
D-lactate
Intestinal Mucosal Barrier
