摘要
自制的铂丝电极(0.3mm)经铂化处理后,将葡萄糖氧化酶(GOD)与单体吡咯用循环伏安法共聚合到电极上,铂化后电极表面积的增大增加了固定的酶量,同时也增大了电极对H2O2的催化能力,使电极对葡萄糖的响应电流增大到7.5mA/(mol·L-1)。还测试了聚合液中酶浓度、pH值和聚合时间等因素对电极特性的影响。
Glucose oxidase(GOD) and pyrrole were electrochemically polymerized onto the platinized platinum wire electrode. Platinization of the platinum wire can greatly enhance the surface area and the catalytic activity of H2O2, which result in largely improving the response of the microenzyme electrode to glucose.
出处
《华东理工大学学报(自然科学版)》
CAS
CSCD
北大核心
1998年第2期225-228,共4页
Journal of East China University of Science and Technology
基金
国家自然科学基金
关键词
葡萄糖氧化酶
电化学聚合
吡咯
电极
biosensor
platinized platinum wire electrode
glucose oxidase
electrochemically polymerization
pyrrole