摘要
目的探讨肿瘤坏死因子相关凋亡诱导配体（TRAIL）在内毒素诱导小鼠肝细胞凋亡中的作用。方法内毒素作用于可溶性死亡受体-5（sDR5）封闭TRAIL的小鼠，不同时间检测小鼠血清中的谷丙转氨酶（ALT）、谷草转氨酶（AST）和乳酸脱氢酶（LDH）的含量；HE染色及AnnexinV、PI双染法流式细胞术检测小鼠肝细胞凋亡；免疫组织化学法及流式细胞术检测小鼠肝细胞和肝细胞膜表面DR5的表达。结果内毒素可以上调肝细胞DR5的表达。DR5封闭TRAIL可显著抑制内毒素引起的小鼠肝细胞凋亡和改善肝细胞损伤。结论内毒素引起疾病过程中TRAIL起重要作用。
Objective To explore the effect of TNF related apoptosis inducing ligand （TRAIL） in apoptosis induced by LPS. Methods After LPS injected mice blecking TRAIL with soluble death receptor 5 （ sDR5 ）, detecting ALT, AST and LDH of mice serum at different times, apoptotie effects of LPS to mice hepatocyte were detected by HE and flow eytometry （FCM） with Annexin V-FITC/PI staining. The expression of DR5 in mice hepatocyte was assayed with immunohistochemistry and FCM. Results Apoptofic effect was promoted by up-regulated DR5 expression on hepatoeyte. Blocking TRAIL with sDR5 markedly ameliorated the hepatoeyte damage and reduced apoptosis. Conclusion These results establish a critical role for TRAIL in apoptosis during disease process of LPS.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2009年第2期151-155,共5页
Chinese Journal of Microbiology and Immunology
基金
河南省教育厅自然科学基金（200510475039）
河南大学自然科学基金（05YBZR022）
