摘要
用带有粟桌酒裂殖酵母钙调索基因的质粒PEC/PCA和质粒PSB6来转化大肠杆菌SB1，扩增培养后破碎离心，上清液经Phenyl-SepharoseCL－4B柱、DEAEcellulose-52柱、SephedesG－75柱分离，得到纯化的表达产物，此产物经SDS—PAGE电泳鉴定和对环核苷酸：酷酶（cyclicnucleotidephosphodiesterase，PDE）活性的激活鉴定，确定为酵母钙调素，且其分产量小于猪脑钙调素。
The plasmid PEC/PCAM carrying CaM gene of S. Pomhe and the PLaswid pSB6 carrying ly-sis gene were transfered into E. coli stain SB1. SB1 was amplified , then lysed and cen-trifuged. The smprtanent was brought to Phenyl - Sephaare CL - 4B, DEAE Cellulose - 52,SePhedex G- 75 coumn separately, the purified product was obtained. This product wa as-sayed by SDS - PAG electrophoresis and by Ca2+ - dependent Phos phediesterase, it wasverified that the product was CaM, and its moecular weight is lower than Porcine brainCaM.
出处
《微生物学杂志》
CAS
CSCD
1998年第1期10-13,共4页
Journal of Microbiology
关键词
粟酒裂殖酵母
钙调素
基因
表达产物
分离
纯化
Schizosaccharomyces pombe
CaM gene
expression product
purification and isolation
