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人巨细胞病毒在体外新生乳鼠海马神经细胞中的增殖能力 被引量：2

Proliferative Ability of Human Cytomegalovirus in Hippocampal Nerve Cells of Neonatal Rats Infected in Vitro
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摘要 目的探讨人巨细胞病毒(HCMV)AD169株在新生乳鼠海马神经细胞体外培养物感染情况及其是否存在增殖能力。方法用人胚成纤维细胞悬液培养增殖,按病毒致细胞病变效应终点稀释法以24～48 h出现(+++)～(++++)的病毒悬液在人胚成纤维细胞上滴定半数组织细胞感染量为5×107L-1,将病毒分别接种在SD大鼠长成单层的新生乳鼠海马48 h神经细胞培养物上,每天显微镜观察记录,采用免疫组织化学方法检测神经细胞培养物上巨细胞病毒早期蛋白,间接免疫荧光检测HCMV pp65蛋白,透射电镜方法观察和鉴定HCMV在神经细胞内的感染情况。结果接种病毒的海马神经细胞在感染后第1天即可观察到特征性细胞病变效应,随着时间的延长,细胞裂解液化消失;光镜下,免疫组织化学可见巨细胞病毒产生早期蛋白表达于受感染的培养海马神经细胞中,呈棕黄色颗粒;HCMVpp65蛋白免疫荧光检测亦为阳性,阴性对照未见任何阳性信号;透射电镜可见新生鼠大脑海马神经元胞质中有直径为80～120 nm病毒颗粒。结论HCMVAD169株能够感染乳鼠海马神经细胞,并具有一定的增殖能力。 Objective To explore infectious of human cytomegalovirus （HCMV） AD169 in neonatal marine hippocampal nerve cells in vitro and whether they have proliferative ability. Methods They were proliferatived with human embryonic fibroblasts（HF） cells,eytopathic effect was （ ＋ ＋ ＋ ） - （ ＋ ＋ ＋ ＋ ） within 24 -48 hours,and 5 × 10^7 L^-1 cell suspension of the HCMV AD169 strain was inoculated onto monolayer nerve cell media of SD neonatal rat hippocampal nerve cells. Cytopathic effect was observed each every day with microscope ,early HCMV protein and pp65 protein were detected by immunohistochemistry and indirect immunofluorescent;the virus titers of cultured supernatant neonatal rats hippocampal nerve cells were determined, and HCMV particles in the nerve cells were observed by electron microscopy. Re- suits Characteristic cytopathic effect was observed on the first day of inoculation and this cytopathic change aggravated as inoculation time prolonged. HCMV early proteins were found using immunohistochemistry, they were buffy panicles in light microscope. HCMV characteristic protein pp65 was detected by indirect immunofluorescent examination and the controls were negative. HCMV particles were observable in the cytoplasm and karyon of the nerve cells under the election microscopy and the diameter of panicles was 80 - 120 nm. Conclusion HCMV can infect neonatal murine hippocampus nerve cells in vitro and has certain proliferative ability.
作者 杨李 吴德 金岚 李晓燕 唐久来
机构地区 安徽医科大学第一附属医院小儿神经康复中心
出处 《实用儿科临床杂志》 CAS CSCD 北大核心 2009年第4期298-300,共3页 Journal of Applied Clinical Pediatrics
基金 国家自然科学基金项目资助(30672270)
关键词 巨细胞病毒感染 病理学 海马 细胞学 大鼠 cytomegalovirus infection pathology hippocampus cytology rat
分类号 R722.1 [医药卫生—儿科]
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参考文献12

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同被引文献22

  • 1王志勇,张循善,王红梅（校对）.核黄素光化学法灭活病原体的研究进展[J].临床输血与检验,2006,8(2):152-154. 被引量：8
  • 2蔡亦红,高荣保,王明丽.人巨细胞病毒增殖性感染新生乳鼠大脑皮质神经细胞[J].安徽医科大学学报,2006,41(3):237-240. 被引量：3
  • 3姚娟,方草晖,王明丽.人巨细胞病毒先天性潜伏感染老龄小鼠肺组织的实验研究[J].中华微生物学和免疫学杂志,2007,27(4):349-352. 被引量：2
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  • 5Ruane PH, Edrich R, Gampp D, et al. Photochemical inactivation of selected viruses and bacteria in platelet concentrates using riboflavin and light[J]. Transfusion,2004,44(6):877- 885.
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  • 9Li LQ,Tan JJ,Zhou Y. Does congenital cytomegalovirus infection lead to hearing loss by inducing mutation of the GJB2 gene[J].Pediatric Research,2013,(02):121-126.
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实用儿科临床杂志
2009年 第4期

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