摘要
目的通过大鼠心肌缺血再灌注损伤的动物模型,分析CD4+T细胞在心肌组织损伤中的作用。方法结扎大鼠冠状动脉左前降支45min,随后恢复再灌的方法,制作缺血再灌损伤的动物模型,随机分为再灌注0、2、6、9、12h组及相应的对照组。II导联心电图及TTC确定模型,组织病理学观察心肌细胞的损伤情况,免疫荧光染色计数浸润的炎性细胞,半定量PCR进一步验证各型T细胞的表达。结果心肌的梗死面积与心肌缺血再灌时间成正相关,至观察结束未出现峰值;组织中浸润的中型粒细胞和T细胞分别在2h和12h有峰值出现,但CD4+T/CD3+T的比率几乎保持不变;观察所见CD4+T细胞是组织中存在最多的T细胞。结论大鼠缺血再灌注损伤中,心肌组织中浸润的CD4+T细胞作为主要的效应细胞,参与了持续稳定的心肌损伤过程。
Objective To investigate the role of infiltrating CD4^＋T cells in a rat model of ischemia-reperfusion injury（IRI）.Methods A IRI model was set up by ligation of the descending anterior branch of coronary artery for 45 min,followed by various times of reperfusion.The rats were randomly divided into five groups according to different reperfusion time of 0,2,6,9,12 h,respectively.The sham-operated rats at those time points as controls.Myocardial dysfunction and tissue injury were confirmed by lead II ECG and TTC and was further evaluated by histology.The changes of amount of infiltrating cells were detected with anti-FITC staining,and the amount of different types of T cells after different reperfusion time durations was further confirmed by semi-PCR.Results The area of myocardial infarction was increased following the lasting reperfusion,and its peak did not occur in this experiment,whereas,the infiltrating cells peaked at 2 h for PMNs and 12 h for T cells;the ratio of CD4^＋T /CD3^＋T remained unchanged.CD4^＋T cells were the major type of T cells altered during IR in the myocardial tissue.Conclusion CD4^＋T cells may represent one of the major effector cells in the rat myocardial IRI,and are involved in the persistent and progressive myocardial injury.
出处
《中国实验动物学报》
CAS
CSCD
2009年第1期65-70,F0003,I0008,共8页
Acta Laboratorium Animalis Scientia Sinica
