摘要
目的探讨仿生诱导骨促进兔脊柱融合的可行性。方法成骨诱导化培养兔脂肪基质细胞、制备释放重组人骨形成发生蛋白（rhBMP）-2的壳聚糖／胶原蛋白／B磷酸三钙基质，联合生物凝胶＋动态种植，构建仿生诱导骨；兔腰4～5横突间融合模型移植4组，A1仿生骨诱导骨；A2自体髂骨；B1rhBMP-2的复合基质；B2空白支架。行组织学、免疫组织化学、新骨及诱导因子定量分析、手动触检，生物力学检查。结果A1组骨性融合能力最强，融合率、新骨面积、BMP含量及力学指标均高于其他组，差异有统计学意义（P〈0．05）；A2组次之，B1组新骨生长不明显，融合相对延缓。B2组未融合。结论仿生诱导骨能强化骨诱导、传导效应，超越自体骨修复效果，促进脊柱融合。
Objective To investigate the feasibility of mimetically osteoinductive bone in promoting spinal fusion of rabbits. Methods With aid of biological gels compound and dynamic （ oscillating pattern） seeding,three-dimensional construction of mimetically osteoinductive bone was made by implanting adipose derived stroma] cells （ADSCs） into rhBMP-2 mediated Cs-Col-β-TCP matix. Lumbar 4-5 intertranverse process fusion was established in New Zealand rabbits. In group A1 the rabbits were transplanted by mimetically osteoinduetive bone,those in group A2 by bone autograft,those in group B1 by rhBMP- 2 mediated Cs-Col-β-TCP matix,and those in group B2 by Cs-Col-β-TCP matix as blank scaffold. Histology, immunohistochemistry, new bone ratio, BMP quantification, and palpation and biomechanical examinations were performed at different time points. Results Compared with other groups, group A1 displayed remarkable fusion capacity with statistical significance according to the data of new bone ratio, BMP content and biomechanical tests （P 〈 0.05）. Conclusion Enhanced osteogenic potential can be achieved by using mimetically osteoinductive bone, and spinal fusion can be promoted with concord pattern of osteoinductive and osteoeonductive effects.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第3期352-354,共3页
Chinese Journal of Experimental Surgery
关键词
脊柱融合
仿生诱导骨
脂肪基质细胞
壳聚糖
胶原蛋白
Spinal fusion
Mimetically osteoinductive bone
Adipose derived stromal cells
Chitosan
Collagen
