摘要
将拟南芥种子点种于添加有不同浓度CdCl2的培养基中处理2周,移苗时CdCl2的胁迫即解除。低浓度CdCl2促进拟南芥种子的萌发。CdCl2为0.5mg·L-1时萌发率最高(为97.21%)。随着CdCl2浓度的继续增加,种子萌发率即逐渐下降。幼苗期和抽薹期分别提取叶DNA,采用甲基敏感扩增多态性(MSAP)技术分析其基因组DNA甲基化的结果显示,总的来说,随着CdCl2浓度的增加,甲基化程度增高。
The seeds of Arabidopsis thaliana were cultured on the mediums with different concentrations of CdCl2 for two weeks, then the seedlings were transplanted on the mediums without CdCl1. The results showed that low concentrations of CdCl2 could enhance the germination rate of Arabidopsis, the highest germination rate of Arabidopsis treated with 0.5 mg·L^-1 CdCl2 was 97.21%. There was a negative correlation between the concentration of CdCl2 and the germination rate. The total genomic DNAs in leaves at seedling stage and bolting stage were isolated and analyzed by the method of methylation-sensitive amplified polymorphism （MSAP）, the results showed that the methylation degrees were increased with the increase of CdCl2 concentration.
出处
《植物生理学通讯》
CSCD
北大核心
2009年第2期115-118,共4页
Plant Physiology Communications
基金
河南大学校内基金(06ZDZR011)
关键词
拟南芥
萌发率
MSAP
甲基化
Arabidopsis thaliana
germination rate
MSAP
methylation
