摘要
从银杏外种皮中提取含94.8%银杏酚的提取物,用氯仿溶解提取物,并加适量乳化剂烷基酚聚氧乙烯醚,配制含12.6%银杏酚的液剂,称为银杏酚液剂。用水稀释银杏酚液剂50、100、150和200倍,用稀释液浸泡苏州青青菜的菜叶,喂饲菜青虫三龄、四龄幼虫。用叶碟法测定银杏酚对菜青虫的拒食活性。结果表明,银杏酚对菜青虫三龄、四龄幼虫具有明显的拒食活性,拒食率达30.14%～85.3%。稀释浓度在150倍以下,对三龄幼虫拒食效果较好,处理后3天拒食作用最强,对三龄幼虫的拒食作用优于四龄幼虫。同时讨论了银杏酚对菜青虫拒食活性的机理。银杏酚对菜青虫拒食活性,是由于银杏酚抑制了酪氨酸酶等酶的合成,影响了菜青虫的蜕皮和生长发育,使其表现为拒食和发育为超龄幼虫,多数不能形成蛹而致死。
The ginkgo exocarp extract containing 94.8 % of ginkgo phenol was dissolved in CHCl3. A little amount of emulsifying agent alkylphenol ethoxylate was added to prepare a liquid containing 12.6 % of ginkgo phenol. The liquid was diluted with 50, 100,150 ,and 200 times of water. The leaves of Breassica chinensis Linn. were soaked with the diluted solutions to feed larvas of cabbage caterpillar at the ages of 3 and 4 years old. The antifeeding rate was up to 30.14 % - 85.30 %. The diluted solution at 150 times has better effect of antifeeding to larva at 3 years old. The peak value of antifeeding was in the third day after treatment. The effect to 3 years lerva was better than that to 4 years larva. The antifeeding mechanism of ginkgo phenol to cabbage caterpillar was discussed. The antifeeding reasons of ginkgo phenol to cabbage caterpillar are that ginkgo phenol can inhibit synthesis of enzymes such as tyrosinase, and affect molting and growth of cabbage caterpillar. It shows antifeeding and developing to supernumerary larva. So most of them can not become pupas and then die.
出处
《生物质化学工程》
CAS
2009年第1期13-16,共4页
Biomass Chemical Engineering
关键词
银杏酚
菜青虫
拒食活性
ginkgo phenol
cabbage caterpillar
antifeeding activity
