摘要
目的探讨直肠癌旁移行粘膜的生物病理学性质。方法应用组织化学和免疫组织化学方法观察34例直肠癌旁粘膜中p53、p21蛋白表达及其与粘蛋白改变的关系。结果 29.4%(10/34)直肠癌旁移行粘膜腺体杯状细胞胞浆内存在p53表达,其范围不超出远端4cm肠管;p21在26.5%(9/34)癌旁移行粘膜腺体柱状细胞头内存在表达,其范围不超出2cm肠管。结论直肠癌旁移行粘膜中存在p53失活及ras激活,系不稳定的癌前期病变,直肠癌远端粘膜粘蛋白异常改变及分子遗传学异常范围在4cm以内。
Objectives To study the biopathologic characteristics of the transitional mucosa adjacent to rectal carcinoma. Methods 34 cases were subjected to mucin histochemical and immunohistochemical study to observe the expression of p53 and p21 protein in the transitional mucosa and its relationship to the mucin change. Results Expression of p53 protein was found in 29.4% (10/34) of distal transitional mucosa in the cytoplasm of goblet cells. Its positive staining was with 4cm distance from carcinoma margin. Overex- pression of p21 protein was found in 26.5% (9/34) of distal transitional mucosa in cytoplasm of crypt cells. Its positive staining was with 2 cm distance from carcinoma margin. Conclusion There are aberrant genetic alternations in transitional mucosa adjacent to rectal carcinoma, providing further evidence that transitional mucosa is an unstable premalignant change. The aberrant mucin change and genetic alterna- tions in distal mucosa of rectal cancer is with 4cm.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第1期38-39,共2页
Chinese Journal of Experimental Surgery
关键词
直肠肿瘤
肠粘膜
P53基因
P21基因
TM
Rectal neoplasms
Intestinal mucosa
Protein p53
Protein p21
Immuno-histochemistry
