摘要
选用白茅、稻草、大米草、黑麦草、高羊茅5种牧草为试验材料,在预处理阶段采用稀酸预处理、稀碱预处理、高温热水预处理、酸催化高温热水预处理,然后又进行纤维素酶的水解试验。结果表明:1)高温热水预处理方法在预处理阶段除稻草外,其他牧草产生的单糖都以甘露糖为主,且在酶解过程中产生的单糖虽以葡萄糖为主,但其最高酶解率仅为6.90%。而稀酸预处理、稀碱预处理、酸催化高温热水预处理方法的效果均明显优于高温热水预处理方法,都比较适宜于利用牧草生产清洁燃料乙醇的预处理过程中;2)针对不同的牧草其最适的预处理方法也各不相同,白茅、大米草的最适预处理方法为稀酸预处理;稻草的最适预处理方法为酸催化高温热水预处理;黑麦草和高羊茅的最适预处理方法为稀碱预处理。
Five species of forages （Imperata cylindrical, rice straw, Common cordgrass, Lolium perenne and Festuca arundinacea） with potentional value to produce biofuels were selected to test. The forages were treated by using dilute acid, dilute alkaline, high temperature and hot water, and acid-catalyzed hydrolysis with high-temperature and hot water respectively during the pretreatment stage. Then the hydrolysis was followed by using eellulase. The pretreatment with high temperature and hot water is found as an inappropriate method comparing with other methods because mannose is produced as the main monosaccharide in the forages except rice straw. And glucose is produced at enzymatic hydrolysis stage with the highest hydrolysis rate of only 6.90o//oo. Then the suitable pretreatments for ethanol production of the forages are dilute acid, dilute alkaline, acid-catalyzed hydrolysis with high temperature and hot water. And the best pretreatment is different among these forages with dilute acid for Imperata cylindrical and Arundodonax Linn, acid-catalyzed hydrolysis with high temperature and hot water for rice straw, dilute alkaline for Common cordgrass and Festuca arundinacea.
出处
《贵州农业科学》
CAS
北大核心
2009年第1期111-115,119,共6页
Guizhou Agricultural Sciences
基金
国家高技术(863计划)专题项目"典型草畜动态信息及其退化特征高(多)光谱遥感快速检测关键技术研究"(2007AA10Z231)
关键词
牧草
稀酸预处理
稀碱预处理
高温热水预处理
酸催化高温热水预处理
纤维素酶
forage
pretreatment with dilute acid
ture and hot water
acid-catalyzed pretreatment with high pretreatment with dilute alkaline
pretreatment with high temperatemperature and hot water
cellulase
