摘要
目的探讨过度机械通气致肺损伤后,炎症因子TNF-α、IL-1β及NF-κBp65在肺组织中不同时间表达的变化规律。方法将51只雄性新西兰大白兔分为正常组、对照组、实验组。利用呼吸机建立过度机械通气致肺损伤模型。应用免疫印迹技术观察兔急性肺损伤后肺组织中TNF-α、IL-1β及NF-κBp65的表达,并用凝胶图像处理系统进行分析,所得数据经SPSS 10.0统计软件处理。结果正常组与各对照组之间比较,均无明显差异(P>0.05)。TNF-α在损伤后0h表达即增加,1h达高峰,以后逐渐下降,而在24h后又有反弹性增高;其中在0.5、24、48h组有差异性(P<0.05),1、3h组有显著差异性(P<0.01)。IL-1β在损伤后0.5h逐渐增加,3h达高峰,以后逐渐下降,其中在1、6、12、24h组有差异性(P<0.05),3h组有显著差异性(P<0.01)。NF-κBp65在损伤后0.5h逐渐增加,6h达高峰,以后逐渐下降,其中在1、12h组有差异性(P<0.05),3、6h组有显著性差异(P<0.01)。结论TNF-α、IL-1β及NF-κBp65在过度机械通气致VILI发病中有重要作用;TNF-α、IL-1β及NF-κBp65在损伤早期即表达,且在时间上有一定的变化规律,可为损伤时间的推断提供参考。
Objective To investigate the expression of NF-κBp65, TNF-α and IL-1β in lung issue after high mechanical ventilation. Methods Building acute lung injury model with high mechanical ventilation, fifty-one male new zealand white rabbits were randomly divided into normal group ,control group and experimental group. The expression of NF-κBp65, TNF-α and IL-1β were observed using western blot and analyzed by gel image processing system, then all data were dealt with SPSS 10.0 statistical software. Results There were no significant differences between normal group and control groups （ P 〉 0.05 ）. The expression of TNF-α increased immediately after high mechanical ventilation, one hour later to the peak, then decreased gradually, but there was increased significantly after 24 hours. Statistic analysis indicated that there were obvious differences at 0.5,24,48 hours （ P 〈 O. 05 ） and greater differences at 1,3 hours （ P 〈 0.01 ） between control group and experimental group. The expression of IL-1β increased at half an hour after high mechanical ventilation, three hours later to the peak, then decreased gradually. Statistic analysis indicated that there were obvious differences at 1,6, 12,24 hours （ P 〈 0. 05 ） and greater differences at 3 hours （P 〈0.01 ）between control group and experimental group. The expression of NF-κBp65 increased at half an hour after high mechanical ventilation, six hours later to the peak, then decreased gradually. Statistic analysis indicated that there were obvious differences at 1,12 hours （P 〈 0. 05 ） and greater differences at 3,6 hours （P〈0. 01）between control group and experimental group. Conclusion The results suggest that NF-κBp65, TNF-α and IL-1β may play a major role in ventilator induced lung injury. Furthermore, NF-κBp65, TNF-α and IL-1β can be used as markers for trauma time determination because of their early expressed and time regularity.
出处
《中国法医学杂志》
CSCD
北大核心
2009年第1期33-35,共3页
Chinese Journal of Forensic Medicine
