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CpG-ODN对伤寒Ty21a疫苗诱导的细胞免疫应答的作用

Effect of CpG-ODN on Cellular Immune Response Induced by Typhoid Ty21a Vaccine
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摘要 目的探讨CpG-ODN对伤寒Ty2la疫苗诱导的细胞免疫应答的作用。方法分别用含10、30及50μgCpG-ODN的伤寒Ty21a疫苗免疫小鼠,各剂量组分别于第0、14和28d灌胃免疫3次,末次免疫后1周,采集小鼠脾细胞。流式细胞仪检测脾细胞MHC-Ⅱ类分子和CD40分子的表达;培养脾细胞,用ELISA法检测脾细胞培养上清液中IFN-γ和IL-10的水平。结果不同剂量CpG-ODN的疫苗免疫组脾细胞CD40和MHC-Ⅱ类分子的表达水平均高于阳性对照组,且随着CpG-ODN剂量的增加,表达增强。加入不同剂量CpG-ODN的疫苗免疫组脾细胞培养上清液中IFN-γ和IL-10的水平均升高,且IL-10随着IFN-γ分泌水平的增加而降低。结论CpG-ODN可提高伤寒Ty21a疫苗诱导的Th1型免疫应答水平。 Objective To explore the effect of CpG-ODN on cellular inmune response induced by typhoid Ty21a vaccine. Methods NLH mice were immunized with typhoid Ty21a vaccines containing 10, 30 and 50 μg of CpG-ODN respectively, by oral route on days 0, 14 and 28 d. The splenocytes were collected one week after the third immunization and determined for expressions of MHC-Ⅱ and CD40 molecules by flow cytometry, then cultured and determined for IFN-γ and IL-10 levels in culture supernatant by ELISA. Results The expression levels of MHC- Ⅱ and CD40 molecules in the splenocytes of mice immunized with typhoid Ty21a vaccines containing various concentrations of CpG-ODN were significantly higher than those of positive control, and increased with the increasing CpG-ODN concentration. The IFN-γ and IL-10 levels in culture supernatant of splenocytes of mice immunized with typhoid Ty21a vaccines containing various concentrations of CpG-ODN increased significantly, and the IL-10 level decreased with the increas- ing IFN-γ level. Conclusion CpG-ODN enhanced the Thl immune response induced by typhoid Ty21a vaccine.
作者 汤贤英 孙万邦 罗军敏 宋明英 杜联峰
机构地区 合肥医学院免疫教研室
出处 《中国生物制品学杂志》 CAS CSCD 2009年第2期162-164,共3页 Chinese Journal of Biologicals
基金 合肥医学院硕士启动基金(F-139)
关键词 CpG—ODN 佐剂 伤寒Ty21a疫苗 细胞免疫应答 CpG-ODN Adjuvant Typhoid Ty21a vaccine Cellular immune response
分类号 R392.1 [医药卫生—免疫学]
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参考文献12

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  • 2Kilhamn J, Lundin SB, Brevinge H, et al. T-and B-cell immune responses of patients who had undergone colectomies to oral administration of Salmonella enterica serovar typhi Ty21a vaccine. Clin Diagn Lab Immunol, 2003, 10(3): 426-430.
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二级参考文献11

  • 1Weeratna R, Comanita L, Davis HL.CPG ODN allows lower dose of antigen against hepatitis B surface antigen in BALB/c mice[J].Immunol Cell Biol, 2003,81(1):59-62.
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  • 5Kilhamn J, Lundin SB, Brevinge H,et al. T- and B-cell immune responses of patients who had undergone colectomies to oral administration of Salmonella enterica serovar Typhi Ty21a vaccine[J].Clin Diagn Lab Immunol, 2003,10(3):426-430.
  • 6Pal S, Davis HL, Peterson EM,et al.Immunization with the Chlamydia trachomatis mouse pneumonitis major outer membrane protein by use of CpG oligodeoxynucleotides as an adjuvant induces a protective immune response against an intranasal chlamydial challenge[J].Infect Immun, 2002,70(9):4812-4817.
  • 7Brunner C, Seiderer J, Schlamp A,et al. Enhanced dendritic cell maturation by TNF-alpha or cytidine-phosphate-guanosine DNA drives T cell activation in vitro and therapeutic anti-tumor immune responses in vivo[J].J Immunol, 2001,165(11):6278-6286.
  • 8Gantner F,Hermann P,Nakashima K,et al.CD40-dependent and-independent activation of human tonsil B cells by CpG oligodeoxynucleotides[J].Eur J Immunol,2003,33(6):1576-1585.
  • 9Krieg AM. CpG motiofs in bacterial DNA and their immune effects[J].Annu Rev Immunol, 2002,20:709-760.
  • 10Wang Y, Krieg AM. Synergy between CpG- or non-CpG DNA and specific antigen for B cell activation[J]. Int Immunol,2003,15(2):223-231.

共引文献5

中国生物制品学杂志
2009年 第2期

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