摘要
目的探讨一次长时运动及耐力运动对骨骼肌线粒体mtNOS及COXⅣ、SOD活性的影响。方法一次长时运动及耐力运动分别选用雄性SD大鼠42只,随机分为7组:对照组;最后一次运动后即刻组、3 h组6、h组、12 h组、18h组、24 h组。提取腓肠肌线粒体,分别检测线粒体mtNOS及COXⅣ、SOD的活性。结果一次急性运动后同安静组比较:骨骼肌线粒体COXⅣ活性在运动后即刻、运动后3 h6、h组明显升高;mtNOS活性在运动后6 h1、2 h、18 h、24 h组明显升高;SOD活性在运动后即刻3、h6、h1、2 h组明显升高。耐力运动后同安静组比较:线粒体COXⅣ活性在运动后各组均升高;mtNOS活性在运动后6 h1、2 h1、8 h、24 h组有明显升高;SOD活性在运动后18 h2、4 h组明显升高。结论两种运动形式均可以提高mtNOS、SOD及COXⅣ的活性,主要通过NOS/COXⅣ通路介导实现。耐力运动可以增强线粒体呼吸功能,促进线粒体生物发生。
Objective To explore the hypothesis that phasic changes of Mito Nitric Oxide Synthase （mtNOS）, Superoxide Dismutase （SOD）and Complex Ⅳ （COX Ⅳ） are related to acute exercise and endurance training. Methods We used two kinds of exercises： acute exercise（AE） and endurance training（ET）. Rats were randomly divided into 7 groups： at rest（C1 ） , Oh group （tested immediately after swimming） ,3 h, 12 h, 6 h, 18 h, 24 h after the last swimming bout respectively. Mitochondria were isolated and activity of enzymes were measured. Results In AE the activity of NOS increased firstly and then decreased whereas the activity of NOS increased at all time courses in ET, it is the same of the activity of COX Ⅳ. The activity of SOD increased at 0 h, 3 h, 6 h, 12 h in AE but only increased at 18 h, 24 h in ET. Conlusion Exereises can increase NOS, SOD and COX Ⅳ activity, mainly through NOS/COX Ⅳ pathway, which inverse modulate the function of respiratory chain and the rnitochondrial biogenesis.
出处
《西安体育学院学报》
CSSCI
北大核心
2009年第1期64-66,106,共4页
Journal of Xi'an Physical Education University
基金
上海市体育局资助(44030750)
上海大学优秀青年教师科研专项基金
上海大学创新基金资助项目
