摘要
目的:观察不同月龄新西兰大白兔椎间盘组织中碱性成纤维细胞生长因子(bFGF)和血管内皮生长因子(VEGF)的表达,探讨其与椎间盘退变的关系。方法:40只健康新西兰大白兔,1、3、6、9、12、18、24、30月龄各5只,每只取椎间盘(L5/6、L6/7和L7/S1)组织制作石蜡切片,行HE染色观察各月龄椎间盘组织结构情况,行免疫组织化学染色检测VEGF和bFGF的表达情况,应用图像分析系统测定染色阳性细胞的累积光密度(integrated optical density,IOD)。结果:HE染色显示,低月龄(1、3、6月龄)组椎间盘组织增生活跃,软骨终板均可见血管长入,尤其是1月龄组;中间月龄(9、12月龄)组椎间盘结构完整清晰,未见血管;18月龄组椎间盘出现退变,24月龄组椎间盘退变加重,30月龄组椎间盘结构破坏及老化严重,24、30月龄组椎间盘血管重新长入。免疫组化检查显示,bFGF和VEGF的IOD值在1月龄组表达最高(分别为38.22±14.13和57.67±15.36),随后逐渐下降,12月龄组表达最低(其IOD值分别为7.47±4.08和12.41±3.74),以后随月龄增加表达逐渐增多,30月龄组bFGF和VEGF的IOD值分别为27.93±11.64和37.54±7.32。低月龄组和高月龄组bFGF和VEGF的IOD值与中间月龄组比较均有显著性差异(P<0.01)。结论:兔椎间盘的结构及血管的发生都随月龄增加发生相应变化;bFGF和VEGF的表达也随月龄增加发生相应改变,两者变化具有相似性。
Objective：To investigate the expression of the basic fibroblast growth factor（bFGF） and vascular endothelial growth factor （VEGF） in intervertebral disc tissue of rabbits at different months and find out the relationship between bFGF and VEGF and degeneration of the intervertebral disc.Method：Sections from intervertebral discs（L5/6,L6/7,L7/S1） tissues of 40 rabbits（5 in each of 1,3,6,9,12,18,24 and 30 months old） were obtained and stained by haematoxylin eosin（HE） and immunohistochemical staining.The disc texture and structure was observed under HE staining,the expression of VEGF and bFGF were tested under immunohistochemistry.The integrated optical density（IOD） of staining-positive cell was processed by image analyzing system with higher the IOD value, higher expression of VEGF and bFGF.Result： Under HE staining,the disc tissue progressed actively in early month group（1,3,6 months） with blood vessel ingrowth into end plate,which was visualized specially in 1 month group;while blood vessel ingrowth into end plate was not visualized in mid-month groups （9,12 months） with disc structure keeping intact.Disc degeneration was evidenced in 18- month group,which became severe in 24-month group,while disc structure proceeded into destruction in 30- month group,and blood vessel reingrowth can be seen in 24- and 30-month group.Under immunohistochemistry,IOD value of VEGF and bFGF reached highest point at 1-month group （38.22±14.13 and 57.67±15.36 respectively）,lowest point at 12-month group（7.47±4.08 and 12.41±3.74 respectively）,which progressed gradually with months and reached 27.93±11.64 and 37.54±7.32 at 30-month group respectively.There was significant difference in IOD value among low-month group,mid-month group and high-month group（P〈0.01）.Conclusion：The structure and vascularizations of intervertebral disc vary with month,along with the expression of bFGF and VEGF,which has the similarity.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
北大核心
2009年第2期143-146,I0005,共5页
Chinese Journal of Spine and Spinal Cord
关键词
碱性成纤维细胞生长因子
血管内皮生长因子
椎间盘
兔
Basic fibroblast growth factor
Vascular endothelial growth factor
Intervertebral disc
Rabbit
