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三种培养基对牛体外受精胚胎发育的影响 被引量：1

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摘要 本文比较了三种培养基:(1)B2(LABORATOIRE C.C.D,France)+颗粒细胞(GC)、(2)IVMD-101(FHK,Japan)、(3)ACM(本实验室自配)+胎鼠成纤维细胞(MEF),对牛体外受精胚胎发育的影响。结果显示,以卵裂率(%)来说,第1,2组和第3组分别为65.3±0.05、73.8±0.12及72.7±0.12;以囊胚率(%)来说,第1,2组和第3组分别为33.8±0.05、10.5±0.07及38.9±0.18;而以AB级囊胚率(%)来说,第1,2组和第3组分别为67.6±0.05、60.1±0.04及71.8±0.11。因此,通过统计分析,在我们所研究的三种培养基中,ACM+MEF效果最好。
作者 李晓晨 樊春波 李华 邓卫平 李华威 黄淑帧 曾溢滔
机构地区 上海交通大学上海医学遗传研究所
出处 《上海畜牧兽医通讯》 2009年第1期15-16,共2页 Shanghai Journal of Animal Husbandry and Veterinary Medicine
关键词 体外受精 体外培养 胚胎发育
分类号 S823 [农业科学—畜牧学]
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参考文献14

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同被引文献13

  • 1曾溢滔,张美兰,陈美珏,周霞娣,黄英,任兆瑞,黄淑帧,胡明信,吴学清,高建明,张斌,徐慧如.应用PCR扩增牛SRY序列进行奶牛胚胎性别鉴定[J].中国科学（B辑）,1993,23(4):371-376. 被引量：71
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  • 7Palma GA,Olivier NS,Neumuller C, et al.Effects of Sex-sorted Spermatozoa on the Efficiency of in vitro Fertilization and Ultrastructure of in vitro Produced Bovine Blastocysts. Anatomia, Histologia, Embryologia . 2008
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上海畜牧兽医通讯
2009年 第1期

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