摘要
从江苏地区５个大型种鸡场孵化后期死亡的１６３枚鸡胚及８４羽出壳弱雏中分离鉴定出９９株大肠埃希氏菌，死胚分离率为３６．８１％，出壳弱雏分离率４６．４３％；从其它９个蛋鸡场４７羽疑似大肠杆菌病的病、死鸡的肝、脾、心包、腹水、输卵管、脑、眼中分离鉴定出２３株大肠埃希氏菌，分离率为４８．９４％。分离菌中有６７株对清洁级昆明种小鼠（ＫＭ）具有强毒力。对其进行大肠埃希氏菌Ｏ群抗原鉴定，共有２２种Ｏ型血清型，Ｏ１１、Ｏ８８、Ｏ１１５为优势血清型，Ｏ７８及Ｏ８１各为３株，Ｏ１８、Ｏ２０、Ｏ２６、Ｏ３６、Ｏ８９各为２株，其余为Ｏ３、Ｏ４、Ｏ９、Ｏ３０、Ｏ６０、Ｏ７５、Ｏ９３、Ｏ１０３、Ｏ１１４、Ｏ１３１、Ｏ１３３及Ｏ１４９；未定型菌１１株，占待检菌株的１６．４２％，其中Ｏ３、Ｏ９、Ｏ１８、Ｏ３０、Ｏ８１、Ｏ１１４、Ｏ１３１及Ｏ１４９为首次发现。对２９株不同血清型不同来源的分离菌进行抗生素敏感性试验，结果，所有菌株对头孢唑啉（ＣＦＺ）极度敏感；对其它抗生素的敏感性则各不相同。此外，本试验还对分离菌的生长培养特性，形态特征，生化特性及对ｌｄ鸡的致病性进行了研究。
isolates of Escherichia coli(E coli) were isolated from 163 dead chicken embryoes in late hatching period and 84 one day old weak chicks from 5 chicken breeding farms in Jiangshu areas ,and again,23 isolates of E coli were isolated from 47 chickns representing other 9 chicken farms in this area,with typical lesions of avian colibacilosic The mean isolation percentage of dead embryoes ,ld weak chicks and chickens suspected with colibacilosis were 36 81%,46 43% and 48 94%, respectively 67 strains had high virulence on KM mice Serotyping demonstrated that O11,O88,O115 serogroups were predominant in 22 serogroups,the others were O78,O81,O18,O20,O26,O36,O89,O3,O4,O9,O30,O60,O75,O93,O103,O114,O131,0133 and O149 11 isolates couldn`t be serotyped(16 42%) Among these serogroups,O3,O9,O18,O30,O81 O114,O131 and O149 were firstly discovered in chicken E coli . Antibiotics sensitivity efforts for 29 representative strains revealed that all isolates were extremely sensitive to CFZ,and indefinitely to other antibiotics The article also discussed the the charaterization of culture ,morphology ,biochemistry and pathogenicity of E coli isolates for ld chick
出处
《西南农业学报》
CSCD
北大核心
1998年第1期90-97,共8页
Southwest China Journal of Agricultural Sciences
关键词
大肠埃希氏菌
血清型
抗生素
鸡病
大肠杆菌病
Chicken Esherichia coli Serogroup Antibiotics Sensitivity Pathogenicity
