摘要
在ＫＮＡＵＥＲ－８３０型氨基酸全自动分析仪上，ＯＰＡ柱前衍生法能快速、准确地测定饲料中的氨基酸含量．色谱条件为：流量１ｍｌ／ｍｉｎ，反应时间５ｍｉｎ，柱温４５℃，洗脱液为醋酸钠和甲醇混合液，采用梯度洗脱．饲料中氨基酸含量的变异系数在０．３７％～７．０３％之间．氨基酸回收率除蛋氨酸外，其余都在９５％～１０５％之间．氨基酸含量与峰面积的相关系数在０．９３２９～０．９９９７之间．
OPA precolumn derivatization method can rapidly and accurately measure contents of amino acids in feeds on modular KNAUER Amino Acid Analyzer 830. Chromatographic conditions are as follows: flow rate 1 ml/min, reaction time 5 min, temperature of column 45 ℃,gradient buffer is a mixture of anhydrous sodium acetate and methanol. Coefficients of variation of amino acid content is 0.37%～7.03%. Recoveries of amino acid range from 95% to 105% except methionine. Correlation coefficient between amino acid contents and the peak areas ranges from 0.932 9 to 0.999 7.
关键词
OPA柱前衍生
氨基酸
色谱条件
饲料
OPA precolumn derivatization
amino acid
chromatographic condition
feed
