摘要
目的检测具有不同产胞外多糖(EPS)能力的变异链球菌菌株在不同pH条件下产EPS的能力及其与gtfA、gtfB、gtfC、gtfD表达变化的关系。方法选取变异链球菌(血清型c)临床分离株502(高产糖株)和参考株UA159(低产糖株),以实时定量逆转录聚合酶链反应(real-timeRT-PCR)方法检测在不同pH条件下与变异链球菌产糖能力密切相关的毒力因子gtfA、gtfB、gtfC、gtfD的表达变化。结果在pH5.5条件下,高产糖株和低产糖株gtfA、gtfB、gtfD的表达均有不同程度的升高,而gtfC略降低,高产糖株gtfB、gtfC的表达水平高于低产糖株。结论gtfs的表达水平与变异链球菌的致龋力密切相关。
Objective To determine the expression level of each gtf under different pH cultural conditions and to find the relationship between gtf expression levels with environmental pH in different strains of Streptococcus mutans （S.mutans）. Methods S.mutans form clinical isolation with different extracellular polysaccharides （EPS） producibility and UA159 were selected. Their ability to produce EPS under pH5.5 and pH7 were tested. Then in two strains, the relative quantity of gtfA, gtfB, gtfC, gtfD＇s mRNA which were related to S.mutan＇s ability to produce EPC, were examined by real-time reverse transcription-polymerase chain reaction （real-time RT-PCR） methods under different pH culture condition. Results At pHS.5, expression levels of gtfA, gtfB, gtfD were increased while that of gtfC were decreased in both strains, and that of gtfB, gtfC were higher in strain which produces more ECP. Conclusion The expression levels of gtfs related closely to the cariogenicity of S.mutan.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2008年第6期667-669,共3页
West China Journal of Stomatology
基金
高等学校博士学科点专项科研基金资助项目(20050610075)
关键词
变异链球菌
葡萄糖基转移酶
实时定量逆转录聚合酶链反应
Streptococcus mutans
glucosyl transferases
real-time reverse transcription-polymerase chain reaction
