摘要
根据文献资料,参考人、鸡、鼠等动物的DNA序列,结合本研究的需要,我们设计了一对引物,其上游正向引物P1为5'-GATACGAAAAGACCGCCGAG-3'(对应于鼠ESR基因第4外显子1010-1029),下游反向引物P2为5'—GCACTCTCTTTGCCCAGTTG—3'(对应于鼠ESR基因第4外显于1322-1341).利用该引物对,按照首轮反应94℃5分钟,58℃1分钟,72℃1分钟,随后35个循环按94℃1分钟,58℃1分钟,72℃1分钟的条件,直接从4个猪的品种的DNA样本中扩增出了一个332bp左右的DNA片段,该片段与鼠ESR基因第4号外显子区相对应.据此,我们认为,该片段可能就是猪ESR基因第4号外显子区的一个DNA.片段.由于该区域编码动物雌激素受体的雌激素结合域,所以,对该片段进行系统研究,有助于弄清猪多产性的分子机理,寻找到中国猪多产性的分子标记.
Considered the objection of the present research, a primer pairs were selected with primer according to the DNA sequence data of the human, chicken and rat. The forward primer was 5' -GATACGAAAAGACCGCCGAG-3' (1010-1029 of rac exon 4), 20bp in sum. The reverse primer was 5' -GCACTCTCTTTGCCCAGTG- 3' (1322-1341 of rat exon 4), 20bp in sum. A 332bp fragment was amplified from the sample of 4 pig breeds by this primer pairs in conditions of 94℃ (5minute) ,58℃(1 minute) ,72℃(1 minute)for the first reaction, and 94℃ (1 minute) 58℃(1 minute), 72℃(1 minute)for the followed 35 cycles. It suggest that this DNA fragment may be exon 4 of swine ESR gene. Because the region codes the hor-mone domains of animal ESR, the study on this DNA fragment could help to make clear the molecular mechanism and find the molecular marker of the high prolificacy of Chinese Pig.
出处
《湖北畜牧兽医》
1998年第1期7-8,共2页
Hubei Journal of Animal and Veterinary Sciences
