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原发性羊水过多产妇胎膜和胎盘组织中水通道蛋白8的表达 被引量：8

Expression of aquaporin 8 in human fetal membrane and placenta of idiopathic polyhydramnios
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摘要 目的探讨水通道蛋白8（AQP8）在原发性羊水过多产妇胎膜和胎盘组织中的表达。方法收集2005年10月—2007年5月重庆医科大学附属第一医院和重庆市妇幼保健院行足月剖宫产分娩（孕37～40周）的原发性羊水过多产妇12例为羊水过多组，同期因社会因素行剖宫产分娩的正常产妇12例为对照组。采用RT—PCR技术检测两组产妇胎膜和胎盘组织中的AQPSmRNA表达水平；采用免疫组化技术检测两组产妇胎膜和胎盘组织中的AQP8蛋白表达水平。结果（1）羊水过多组羊膜、绒毛膜和胎盘组织中的AQP8mRNA表达水平分别为0．78±0．13、0．58±0．10、0．86±0．15，对照组分别为0．39±0．07、0．45±0．09、0．34±0．09，羊水过多组各组织中AQP8mRNA表达水平均高于对照组，两组分别比较，差异均有统计学意义（P〈0．05）。（2）羊水过多组羊膜、绒毛膜和胎盘组织中的AQP8蛋白表达水平分别为0．195±0．024、0．170±0．028、0．193±0．024，对照组分别为0．151±0．018、0．156±0．024、0．152±0．023，其中羊水过多组羊膜、胎盘组织中AQP8蛋白表达水平均高于对照组，差异有统计学意义（P〈0．05），而羊水过多组绒毛膜组织中AQP8蛋白表达水平虽高于对照组，但两组比较，差异无统计学意义（P〉0．05）。结论原发性羊水过多产妇羊膜和胎盘组织中AQP8mRNA及蛋白的表达水平均显著高于正常产妇，提示AQP8在产妇羊水量的调节中发挥重要作用。 Objective To determine the expression of Aquaporin 8 （AQP8） in the fetal membrane and placenta of idiopathic polyhydramnios. Methods The amnion, chorion and placenta were collected from 12 term pregnancies with idiopathic polyhydramnios （polyhydramnios group） and 12 term pregnancies who were normal （ control group）. The expression of AQP8 mRNA was detected by reverse transcriptionpolymerase chain reaction （RT-PCR）. The expression of AQP8 protein was detected by immunohistoehemistry. Results The expression of AQP8 mRNA in amnion, chorion and placenta of polyhydramnios group was （0. 78±0. 13） , （0. 58±0. 10） , and （0. 86 ±0. 15） respectively, and that of control group was （ 0. 39±0. 07 ）, （ 0. 45 ± 0. 09 ）, and （ 0, 34 ± 0.09 ） respectively. The expression of AQP8 protein in amnion, chorion and placenta of polyhydramnios group was （0. 195 ±0. 024）, （0. 170 ± 0.028）, and （0. 193 ±0.024） respectively, and that of control group was （0. 151±0.018）, （0. 156 ±0. 024） , and （0. 152 ±0. 023） respectively. In all 3 types of tissues the expression of AQP8 mRNA of polyhydramnios group was higher than that of control group （ P 〈 0. 05 ） . In amnion and placenta the expression of AQP8 protein of polyhydramnios group was also increased compared to that of control group （P 〈0.05 ）, but in chorion the difference in AQP8 protein expression between the two groups was not significant（P 〉 0.05）. Conclusion The expression of AQP8 mRNA and protein is significantly increased in the amnion and placenta of polyhydramnios, suggesting that AQP8 may play an important role in the regulation of amniotie fluid volume.
作者 黄锦 漆洪波
机构地区 重庆医科大学附属第一医院妇产科
出处 《中华妇产科杂志》 CAS CSCD 北大核心 2009年第1期19-22,共4页 Chinese Journal of Obstetrics and Gynecology
基金 国家自然科学基金（30672244）
关键词 羊水过多 胚外膜 胎盘 水孔蛋白质类 Polyhydramnios Extraembryonic membranes Placenta Aquaporins
分类号 R686 [医药卫生—骨科学]
  • 相关文献

参考文献14

  • 1Shioji M, Fukuda H, Kanzaki T, et al. Reduction of aquapofin-8 on fetal membranes under oligohydramnios in mice lacking prostaglandin F2 alpha receptor. J Obstet Gynecol Res, 2006, 32 : 373-378.
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二级参考文献20

  • 1Fang WANG Xue-chao FENG Yong-ming LI Hong YANG Tong-hui MA.Aquaporins as potential drug targets[J].Acta Pharmacologica Sinica,2006,27(4):395-401. 被引量：22
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  • 8Damiano A, Zotta E, Goldstein J, et al. Water channel proteins AQP3 and AQP9 are present in syncytiotrophoblast of human term placenta.Placenta ,2001,22 (8-9) : 776 - 781.
  • 9Wang S, Kallichanda N, Song W, et al. Expression of aquaporin-8 in human placenta and chorioamniotic membranes. Am J Obstet Gynecol,2001,185(5) : 1226 - 1231.
  • 10Wang S, Chen J, Katherine T, et al. Expression of aquaporin-8 and its up-regulation by cyclic adenosine monophosphate in human WISH cells. Am J Obstet Gynecol,2003,188(4) : 997 - 1001.

共引文献10

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引证文献8

二级引证文献28

中华妇产科杂志
2009年 第1期

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