摘要
目的:探讨疏密波促进骨髓间充质干细胞(MSCs)增殖的最佳波段组合,并从分子生物学水平阐明其可能的作用机制,为疏密波临床治疗提供实验依据。方法:体外分离、培养大鼠MSCs,流式细胞仪进行鉴定,MTT法检测疏密波对MSCs增殖的影响。结果:不同波形组合的疏密波均能不同程度地促进MSCs增殖,以C组为最佳,与空白对照组相比差异有统计学意义(P<0.01),但各组组内差异无统计学意义(P>0.05)。结论:疏密波能明显促进MSCs增殖,增殖程度与波形组合有关,以C组的促增殖作用效应最大。
Objective：To investigate the mechanisms and the best combination of dense--sparse waves promoting proliferation of bone marrow mesenchymal stem cells （MSCs） so as to provide experimental foundation for clinical application of dense--sparse waves. Methods： MSCs were obtained from SD rats and identified by FCM. Cell proliferation was detected by MTT assay. Results： All combination of dense--sparse waves significantly promoted MSCs proliferation, with best results in group C. There was significant difference between control group and dense--sparse wave treated groups （P〈0. 01）, but there was no obvious difference within dense--sparse wave treated groups （P〉0.05）. Conclusion： Dense--sparse waves can promote MSCs proliferation. The effect is related to the combination of wave shapes, and group C has the best effect on proliferation.
出处
《中国中医骨伤科杂志》
CAS
2009年第2期16-18,共3页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
福建省卫生厅中医药科技重点项目(编号:WZZG0602)
关键词
骨髓间充质干细胞
疏密波
细胞增殖
Bone marrow mesenchymal stem cells
Dense--sparse waves
Cell proliferation
