摘要
目的:研究二苯乙烯苷(2,3,5,4’tetrahydroxystilbene-2-O-β-D-glucoside,THSG)对体外培养的人乳腺癌MCF-7细胞增殖的作用及对PI3K/Akt信号通路的影响。方法:MTT法检测细胞增殖活性,AnnexinⅤ/PI双标法检测细胞凋亡,Western印迹法检测PTEN、p-PKB、cyclinD1、Bcl-2、caspase3和NF-κB蛋白的表达,RT-PCR检测PI3K上游抑制物PTEN mRNA的表达。结果:1、10和100μmol/L的THSG处理细胞24、48和72h后,明显抑制MCF-7细胞增殖,100μmol/L作用48h的增殖抑制率为41.8%(P<0.05),但低浓度(0.1、0.01μmol/L)THSG出现弱的促增殖作用。1、10和100μmol/LTHSG作用48h后,MCF-7细胞凋亡率分别为(6.25±0.65)%、(5.04±0.83)%和(7.98±0.67)%。1、10和100μmol/LTHSG上调PTEN的蛋白表达量,下调p-PKB和cyclin D1的蛋白表达,均呈剂量依赖性;而对NF-κB、Bcl-2和caspase-3表达却无明显影响。THSG能轻微上调PTEN mRNA水平。结论:THSG可抑制MCF-7细胞增殖,该作用可能是通过抑制PI3K/Akt信号通路实现的。
Objective：To study the effect of 2,3,5,4-tetrahydroxystilbene-2-O-β-D glucoside （THSG） on the proliferation of human breast cancer MCF-7 cells and phosphoinositol-3-kinase/Akt （PI3K/Akt） signaling pathway. Methods： The proliferation of MCF-7 cells was measured with MTT assay and the apoptotic rate of MCF-7 cells was determined by flow cytometry. The levels of PTEN, phosphorylated PKB （p-PKB） , cyclin DI, Bcl-2, caspase-3 and NF-κB protein expression were examined by Western blotting. The expression level of PTEN mRNA was detected by semi-quantitative RT-PCR. Results：THSG 1, 10, and 100 μmol/L obviously inhibited MCF-7 cell proliferation after 24-, 48-, and 72-h treatment. THSG 100 μmol/L decreased the proliferation of MCF-7 cells by 41.8% at 48 h （P 〈0.05）. But THSG at a low concentration （0.1 and 0.01μmol/L） had weak effects in inducing the proliferation of MCF-7 cells. After treatment with 1 , 10, and 100μmol/L of THSG for 48 h, the apoptotic rate of MCF-7 cells was （6.25 ± 0.65） %, （5.04± 0.83 ） %, and （7.98 ± 0.67） %, respectively. THSG 1, 10, and 100 μmol/L upregulated the level of PTEN protein and downregulated the expressions of p-PKB and cyclin D1 in a dose-dependent manner. THSG had no significant effects on the expression of Bcl-2, caspase-3, and NF-κB. THSG induced weak upregulation of PTEN mRNA expression. Conclusion：THSG inhibited the proliferation of MCF-7 cells. The effects may be achieved through depressing PI3K/Akt signaling pathway.
出处
《肿瘤》
CAS
CSCD
北大核心
2009年第1期26-30,共5页
Tumor
