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枯草芽孢杆菌B29菌株防治黄瓜枯萎病的田间效果及安全性评价初报 被引量:39

Evaluation of Biocontrol Efficiency and Security of A Bacillus Subtilis Strain B29 Against Cucumber Fusarium wilt in Field
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摘要 B29菌株是自黄瓜根围土壤中分离到的一株拮抗枯草芽孢杆菌菌株,一年两季4点田间小区试验表明:B29菌株100、250、500倍稀释菌液对黄瓜枯萎病的防效分别为70.3%~88.2%、62.3%~85.9%和54.7%~80.6%;经两年田间大区示范,B29菌株对黄瓜枯萎病防效达84.9%,增产12.57%;同时测定了枯草芽孢杆菌菌剂对大鼠急性经口、经皮毒性,LD50均大于5 000 mg·kg-1;对主要作物的安全性测定结果表明,B29菌株对黄瓜、番茄、菜豆、籽用南瓜4种主要蔬菜作物种子的出苗和幼苗生长发育没有不良影响。 Bacillus subtilis strain B29, against Fusarium oxysporum f. sp. Cucumerinum, was isolated from cucumber rhizosphere. After twice of 4-field-plots experiments, the control efficiencies of 100, 250, and 500 dilution times to cucumber Fusarium wilt were 70. 3 % - 88.2 % , 62.3 % - 85.9 % , and 54.7 % - 80.6 % , respectively. The average efficiency of field trials with B29 was 84.9 % during 2 years and the yield of cucumber increased 12.57 %. The acute toxicity of Bacillus subtilis strain B29 to big mouse through its mouth and skin was examined, and the LD50 was beyond 5 000 mg·kg^-1 The strain B29 to the seedling rate, growth and development with 4 major crops (cucumber, tomato, bean, and seed pumpkin) was safe.
出处 《中国蔬菜》 北大核心 2009年第2期30-33,共4页 China Vegetables
基金 国家农业科技成果转化资金项目(05EFN212300089)
关键词 生物防治 黄瓜枯萎病 枯草芽孢杆菌 安全性 Biocontrol Cucumber Fusarium wilt Bacillus subtilis Security
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