摘要
目的建立复方新斯的明眼用凝胶中维生素B6与有关物质的含量测定方法。方法采用高效液相色谱法，Eclipse XDB—C18色谱柱（4．6mm×150mm，5μm）。流动相：醋酸三乙胺溶液。检测波长：290nm。结果线性范围为1～50μg·mL^-1，r=0．9999（n=5）。低、中、高三个浓度回收率为（100．59±0．57）％，（100．97±0．38）％，（102．20±0．06）％。结论本方法简便快速、专属性好、重复性好、易操作。
OBJECTIVE To establish an HPLC method for determining the content of vitamin B6 and related substances. METHODS A eclipse XBD-C18 column（4.6 mm × 150 mm,5 μm） was used, and the mobile phase（ 1 000 mL） consisted of 0.34 g sodium acetate, 8.25 mL ice acetic acid and 5mL triethylamine. The detective wavelength was 290 nm. RESULTS An excellent linearity（r = 0.999 9, n = 5） was obtained over the range of 1-50μg ·L^-1, the recovery of low, medium and high concentration level was （100.59±0.57）%,（100.97±0.38）% and （102.20±0.06）%,respectively. CONCLUSION This method is sensitive, specific, reproducible and easy to process.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第1期63-65,共3页
Chinese Journal of Modern Applied Pharmacy
