摘要
目的探讨大鼠肾缺血再灌注(ischemia reperfusion,IR)后血清白介素-1β(IL-1β)和肺细胞凋亡的变化。方法将48只Sprague-Dawley大鼠随机平均分为IR组(n=40)和假手术对照组(n=8)。IR组再按照缺血再灌注时间随机分为2 h(IR 2 h组)、6 h(IR 6 h组)、12 h(IR 12 h组)、24 h(IR 24 h组)和72 h组(IR 72 h组),每组8只大鼠。IR组用无损伤动脉夹钳夹大鼠双侧肾蒂45 min制成肾缺血再灌注损伤(renal ischemia reperfusion injury,RIRI)模型。假手术对照组只暴露双肾,不钳夹肾蒂。观察缺血再灌注后2 h,6 h,12 h,24 h和72 h各时间点血清白介素-1β及血清肌酐(scr)水平变化和肺组织细胞凋亡改变。结果假手术对照组血清白介素-1β、血清肌酐水平及肺组织细胞凋亡阳性细胞数均为最低。IR组缺血再灌注2 h后,血清白介素-1β、血清肌酐水平及肺组织细胞凋亡阳性细胞数均逐渐升高,12 h达峰值(IR 12 h组)。肾缺血再灌注12 h,肺组织细胞病理学改变最为严重。结论肾缺血再灌注后,大鼠血清白介素-1β、血清肌酐水平升高及肺组织细胞凋亡阳性细胞数增多。
Objective To investigate the expression of serum interleukin-1β（IL-1β） and the apoptosis of lungs in rats with renal ischemia reperfusion （IR）. Methods Forty-eight Sprague Dawley rats were divided into 6 groups randomly, including IR groups （IR 2 h group, IR 6 h group, IR 12 h group, IR 24 h group, IR 72 h group） and the sham control group. The animal models of IR groups were set up by the operation of 45 minutes bilateral renal pedicle clamping and later reperfusion after 2 h,6 h,12 h,24 h,72 h on Sprague-Dawley rats. Samples for serum and the left lung of each animal were taken. The serum sample was for the analysis of the expression of interleukin-1β, scr by the method of ELISA, and the lung sample for apoptosis and histological examination. Results The expression levels of serum interleukin-1β, ser and apoptosis positive cells of lungs were gradually increased from the sham control group to IR 12 h group. The number of cells in lung underwent apoptosis was obviously increased in IR 12 h group than that in the sham control group and other IR groups. The pathological lesions of lungs were most severe at IR 12 h group. Conclusion The expression levels of serum interleukin-1β, scr and apoptosis positive cells of lungs in rats with renal ischemia reperfusion are increased.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2009年第1期34-36,共3页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
关键词
肺
白细胞介素-1Β
肾
缺血再灌注
lung
interleukin-1β
kidney
ischemia reperfusion （IR）
