摘要
目的：建立稳定的全胰十二指肠移植动物模型。方法：采用原位低温灌洗的方法，获取猪胰腺十二指肠，然后采用不同的方法作同种异体全胰十二指肠移植。供胰放适当位置，采用带有腹腔动脉和肠系膜上动脉的腹主动脉片或腹主动脉段与受体腹主动脉或髂总动脉吻合，门静脉与下腔静脉或髂总静脉吻合，重建血液循环。结果：移植物置腹腔左侧，胰尾向左上，腹主动脉片与腹腔动脉吻合，门静脉与下腔静脉吻合，成功率高，模型稳定。结论：避免血管扭曲、受压，保证吻合口通畅及采用腹主动脉片是移植成功的关键，成活的胰腺可恢复内外分泌功能。
Objectives:To establish whole pancreatoduodenal allotransplantation model. Methods:Outbred white pigs weighting 20~25 kg were used.A whole pancreatoduodenal graft was harversted with the arterial supply derival from a Carrel patch or aortic conduit containing the celiac axis and the superior mesenteric artery and the portal vein.Cooling of the grafts was accomplished with in situ flush with 4℃ preservation solution via a aortic cannula. Results:The proper techniques of porcine pancreatoduodenal allotransplantation is that the Carrel patch is sutured to the aorta,the portal vein is anastomosed to the inferior vena cava by end toside fashing,the graft is placed on the left of desending colon. Conclusions:Avoiding vascalar compress,kinkle and stricture of anastomosis is important for the successful transplantation.The survival pancreas can restore endocring and exocrine function.
出处
《南京大学学报（自然科学版）》
CAS
CSCD
1998年第2期206-210,共5页
Journal of Nanjing University（Natural Science）
基金
中国人民解放军"九五"重点课题
