摘要
干细胞生长因子（Ｓｔｅｍ Ｃｅｌｌ Ｆａｃｔｏｒ， ＳＣＦ）能够刺激造血干细胞生长，并与多种细胞 因子有协同作用．研究中，运用ＰＣＲ手段，从中国人胎肝ｍＲＮＡ中扩增得到编码人ＳＣＦ全 部胞外部分的ｃＤＮＡ．经测序鉴定后，将其克隆入表达载体ｐＲＳＥＴ－Ｃ，并在大肠杆菌ＤＥ３菌 株进行表达．Ｗｅｓｔｅｒｎ印迹法证实表达产物确为人ＳＣＦ，并对其包涵体作了变性、复性的初步 研究．该成果为ＳＣＦ的产品化打下了基础，并将有助于其结构与功能的研究．
Stem cell factor(SCF) can stimulate the growth of stem cells, and has synergetic effect with many other cytokines. The cDNA coding for the whole extracellular portion of human SCF was amplified with PCR technique from the chinese embryonic liver mRNA. After sequencing, cDNA fragment of SCF was cloned into the expression vector PRSET-C and expressed in the E. coli DE3. Western blotting identified that the protein product expressed in E. colt was indeed the human SCF. Denaturing and renaturing of the inclusion body was carried out in present study. This work will found the base of production of SCF by biotechnology, and Promote the study on the relationship between its structure and function.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第2期221-224,共4页
Journal of Fudan University：Natural Science
基金
上海市科委高技术项目
